Background: Extended Spectrum Beta-Lactamase producing E.coli has been increasing over the years. Due to its capability of resisting multiple drugs, patients have higher mortality and morbidity rates and often experience longer length of hospital stay and delayed or misdiagnosis and more costs. Objectives: To determine the prevalence and antimicrobial resistance pattern of Extended Spectrum Beta-Lactamase producing E.coli in human isolates at KCMC, Moshi. Methodology: This study will be a cross-sectional study from April-May 2016. Samples will be collected clinically by using the Standard Operating Procedure. The samples collected will be urine and faecal samples. E.coli will be identified using Biochemical tests and susceptibility antibiotics will be done using disc diffusion methods. Data will be summarized and analysed by using SPSS. Results: A total of 43 (58.9%) out of 73 samples were negative for ESBL producing E.coli. All the positive ESBL samples were more than 70% resistant to the cephalosporin except for amikacin where the positive samples were 60% resistant. Conclusion: The prevalence of ESBL-EC was found to be low. However, the antibiotic resistance pattern of ESBL-EC is significantly high.