Browsing by Author "Beyene, N."

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Accuracy of giant African pouched rats for diagnosing tuberculosis: comparison with culture and Xpert W MTB/RIF
(Researchgate) Mulder, C.; Mgode, G. F.; Ellis, H.; Valverde, E.; Beyene, N.; Cox, C.; Reid, S. E.; Van’t Hoog, A. H.; Edwards, T. L.
Active tuberculosis detection by pouched rats in 2014: more than 2,000 new patients found in two countries
(2016 Society for the Experimental Analysis of Behavior) Poling, A.; Beyene, N.; Valverde, E.; Mulder, C.; Cox, C.; Mgode, G.; Edwards, T. L.
From pests to tests: training rats to diagnose tuberculosis
(ERS 2020) Fiebig, L.; Beyene, N.; Burny, R.; Fast, C. D.; Cox, C.; Mgode, G. F.
From pests to tests: training rats to diagnose tuberculosis
(ERS 2020, 2020) Fiebig, L.; Beyene, N.; Burny, R.; Fast, C. D.; Cox, C.; Mgode, G. F.
2020 is the year of the rat. The rat is the first of the 12 Chinese zodiac signs, and represents spirit, alertness, flexibility and vitality. In respiratory medicine, we may think of rats as vectors for diseases, such as pulmonary forms of hantavirus disease or leptospirosis, and pneumonic plague. Rodent control is thus part of hygiene guidelines and the International Health Regulations. And yet, the rat’s keen sense of smell has led to its incredible career as a living tuberculosis (TB) detector. The TB detection rat journey began with an idea in Tanzania in 2001, roughly 120 years after the TB-causing Mycobacterium tuberculosis was discovered by microscopy and bacterial culture. African giant pouched rats (Cricetomys ansorgei) had already been successfully trained using positive reinforcement to find explosives [1]. So, the question arose if they could do more for humanity by helping to also combat disease. But how would the rats use their noses to find bacteria or otherwise sniff out TB patients? For centuries, olfaction had already informed diagnostics in medical practices, e.g. in detecting metabolic issues such as ketoacidosis or tissue decay such as gangrene [2]. In the Flemish common language, the word tering, which etymologically refers to the smell of tar, is used for TB. The advent of chromatographic techniques allows characterisation of the organic compounds causing odours.
Pouched rats’ detection of tuberculosis in human sputum: Comparison to culturing and polymerase chain reaction
(Tuberculosis Research and Treatment) Mahoney, A.; Weetjens, B. J.; Cox, C.; Beyene, N.; Reither, K.; Makingi, G.; Jubitana, M.; Kazwala, R. R.; Mfinanga, G. S.; Kahwa, A.; Durgin, A.; Poling, A.
Pouched rats’ detection of tuberculosis in human sputum: Comparison to culturing and polymerase chain reaction
(Tuberculosis Research and Treatment, 2012) Mahoney, A.; Weetjens, B. J.; Cox, C.; Beyene, N.; Reither, K.; Makingi, G.; Jubitana, M.; Kazwala, R. R.; Mfinanga, G. S.; Kahwa, A.; Durgin, A.; Poling, A.
Setting. Tanzania. Objective. To compare microscopy as conducted in direct observation of treatment, short course centers to pouched rats as detectors of Mycobacterium tuberculosis. Design. Ten pouched rats were trained to detect tuberculosis in sputum using operant conditioning techniques. The rats evaluated 910 samples previously evaluated by smear microscopy. All samples were also evaluated through culturing and multiplex polymerase chain reaction was performed on culture growths to classify the bacteria. Results. The patientwise sensitivity of microscopy was 58.0%, and the patient-wise specificity was 97.3%. Used as a group of 10 with a cutoff (defined as the number of rat indications to classify a sample as positive for Mycobacterium tuberculosis) of 1, the rats increased new case detection by 46.8% relative to microscopy alone. The average samplewise sensitivity of the individual rats was 68.4% (range 61.1–73.8%), and the mean specificity was 87.3% (range 84.7–90.3%). Conclusion. These results suggest that pouched rats are a valuable adjunct to, and may be a viable substitute for, sputum smear microscopy as a tuberculosis diagnostic in resource-poor countries.
Using giant african pouched rats to detect human tuberculosis: A review
(Pan African Medical Journal) Poling, A.; Mahoney, A.; Beyene, N.; Mgode, G.; Weetjens, B.; Cox, C.; Durgin, A.
Using giant african pouched rats to detect human tuberculosis: A review
(Pan African Medical Journal, 2015-08-31) Poling, A.; Mahoney, A.; Beyene, N.; Mgode, G.; Weetjens, B.; Cox, C.; Durgin, A.
Despite its characteristically low sensitivity, sputum smear microscopy remains the standard for diagnosing tuberculosis (TB) in resource-poor countries. In an attempt to develop an alternative or adjunct to microscopy, researchers have recently examined the ability of pouched rats to detect TB-positive human sputum samples and the microbiological variables that affect their detection. Ten published studies, reviewed herein, suggest that the rats are able to detect the specific odor of Mycobacterium tuberculosis , which causes TB, and can substantially increase new-case detections when used for second-line TB screening following microscopy. Further research is needed to ascertain the rats' ability to detect TB in children and in HIV-positive patients, to detect TB when used for first-line screening, and to be useful in broad-scale applications where cost- effectiveness is a major consideration.