Browsing by Author "Fyumagwa, R. D."

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Cyanobacterial toxins and bacterial infections are the possible causes of mass mortality of lesser flamingos in Soda lakes in northern Tanzania
Fyumagwa, R. D.; Bugwesa, Z; Mwita, M; Kihwele, E.S; Nyaki, A; Mdegela, R. H.; Mpanduji, D.G
Isolation and potential for transmission of mycobacterium bovis at human–livestock–wildlife interface of the Serengeti Ecosystem, Northern Tanzania
(PubMed) Katale, B. Z.; Mbugi, E. V.; Siame, K. K.; Keyyu, J. D.; Kendall, S.; Kazwala, R. R.; Dockrell, H. M.; Fyumagwa, R. D.; Michel, A. L.; Rweyemamu, M; Streicher, E. M.; Warren, R. M.; Helden, P.; Matee, M. I.
Isolation and Potential for Transmission of Mycobacterium bovis at Human–livestock–wildlife Interface of the Serengeti Ecosystem, Northern Tanzania
(DOI) Katale, B. Z.; Mbugi, E.; Siame, K. K.; Keyyu, J. D.; Kendall, S.; Kazwala, R. R.; Dockrell, H. M.; Fyumagwa, R. D.; Michel, A. L.; Rweyemamu, M.; Streicher, E. M.; Warren, R. M.; Helden, P.; Matee, M.
Isolation and potential for transmission of mycobacterium bovis at human–livestock–wildlife interface of the Serengeti Ecosystem, Northern Tanzania
(PubMed, 2015) Katale, B. Z.; Mbugi, E. V.; Siame, K. K.; Keyyu, J. D.; Kendall, S.; Kazwala, R. R.; Dockrell, H. M.; Fyumagwa, R. D.; Michel, A. L.; Rweyemamu, M; Streicher, E. M.; Warren, R. M.; Helden, P.; Matee, M. I.
Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), is a multihost pathogen of public health and veterinary importance. We characterized the M. bovis isolated at the human– livestock–wildlife interface of the Serengeti ecosystem to determine the epidemiology and risk of crossspecies transmission between interacting hosts species. DNA was extracted from mycobacterial cultures obtained from sputum samples of 472 tuberculosis (TB) suspected patients and tissue samples from 606 livestock and wild animal species. M. bovis isolates were characterized using spoligotyping and Mycobacterial Interspersed Repetitive Units-Variable Tandem Repeats (MIRU-VNTR) on 24 loci. Only 5 M. bovis were isolated from the cultured samples. Spoligotyping results revealed that three M. bovis isolates from two buffaloes (Syncerus caffer) and 1 African civet (Civettictis civetta) belonged to SB0133 spoligotype. The two novel strains (AR1 and AR2) assigned as spoligotype SB2290 and SB2289, respectively, were identified from indigenous cattle (Bos indicus). No M. bovis was detected from patients with clinical signs consistent with TB. Of the 606 animal tissue specimens and sputa of 472 TBsuspected patients 43 (7.09%) and 12 (2.9%), respectively, yielded non-tuberculous mycobacteria (NTM), of which 20 isolates were M. intracellulare. No M. avium was identified. M. bovis isolates from wildlife had 45.2% and 96.8% spoligotype pattern agreement with AR1 and AR2 strains, respectively. This finding indicates that bTB infections in wild animals and cattle were epidemiologically related. Of the 24 MIRU-VNTR loci, QUB 11b showed the highest discrimination among the M. bovis strains. The novel strains obtained in this study have not been previously reported in the area, but no clear evidence for recent cross-species transmission of M. bovis was found between human, livestock and wild animals.
Treponema pallidum Infection in the Wild Baboons of East Africa: Distribution and Genetic Characterization of the Strains Responsible
(PLOS ONE) Harper, K. N.; Fyumagwa, R. D.; Hoare, R.; Wambura, P. N.; Coppenhaver, D. H.; Sapolsky, R. M.; Alberts, S. C.; Tung, J.; Rogers, J.; Kilewo, M.; Batamuzi, E. K.; Leendertz, F. H.; Armelagos, G. J.; Knauf, S.
Widespread Treponema pallidum Infection in Nonhuman Primates, Tanzania.
(centers for disease control and prevention.) Chuma, I. S; Batamuzi, E. K.; Collins, D. A.; Fyumagwa, R. D.; Hallmaier-Wacker, L. K.; Kazwala, R. R.; Keyyu, J. D.; Lejora, I. A.; Lipende, I. F.; Lüert, S.; Paciência, F. M.D.; Piel, A.; Stewart, F. A.; Zinner, D.; Roos, C.; Knau, S.
Widespread Treponema pallidum Infection in Nonhuman Primates, Tanzania.
(centers for disease control and prevention., 2018) Chuma, I. S; Batamuzi, E. K.; Collins, D. A.; Fyumagwa, R. D.; Hallmaier-Wacker, L. K.; Kazwala, R. R.; Keyyu, J. D.; Lejora, I. A.; Lipende, I. F.; Lüert, S.; Paciência, F. M.D.; Piel, A.; Stewart, F. A.; Zinner, D.; Roos, C.; Knau, S.
We investigated Treponema pallidum infection in 8 nonhuman primate species (289 animals) in Tanzania during 2015–2017. We used a serologic treponemal test to detect antibodies against the bacterium. Infection was further confirmed from tissue samples of skin-ulcerated animals by 3 independent PCRs (polA, tp47, and TP_0619). Our findings indicate that T. pallidum infection is geographically widespread in Tanzania and occurs in several species (olive baboons, yellow baboons, vervet monkeys, and blue monkeys). We found the bacterium at 11 of 14 investigated geographic locations. Anogenital ulceration was the most common clinical manifestation; orofacial lesions also were observed. Molecular data show that nonhuman primates in Tanzania are most likely infected with T. pallidum subsp. pertenue–like strains, which could have implications for human yaws eradication.