Browsing by Author "Gidamis, A. B."
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Item Comparative evaluation of different methods of extraction and purification used in technical enzyme production from microorganisms(Tanzania J. Agric. Sc) Gidamis, A. B.; Nnko, A.; Shayo, N. B.; Chove, B. E.; Kroner, H. K.Item Comparative evaluation of different methods of extraction and purification used in technical enzyme production from microorganisms(Tanzania J. Agric. Sc, 1998) Gidamis, A. B.; Nnko, A.; Shayo, N. B.; Chove, B. E.; Kroner, H. K.A stlldy was conducted to compare the efficiency of two methods of microbial cell separation and enzyme purification using penicillin-G-acylase (PGA) from Escherichia coli. The efficiency of two methods of ceJ[ separation; centrifugation and cross flow filtration (CFF) were compared. The CFF method was found to have both higher separation efficiency and enzyme yield than centrifugation method. Centrifugation method gave a separation efficiency of 98.5% with enzyme yield of94 % whereas CFFmethod resulted in 100% separation efficiency and enzyme yield of98.8%. The Escherichia coli cells were disrupted by high pressure homogenization (HPH),and the disrupted cells were purified using two different techniques. Technique I was a combination of cross-flow-diafiltration (CFD) , ultrafiltration (UF) and heat/pH-shift treatments. This technique resulted in 47% enzyme yield with a purification factor of 12. Technique II which involved two extraction steps by' aqueous two - phase system (APS) coupled with UF resulted in 62 % enzyme yield with a pu;ificationfactor of 4. Technique I was therefore much better than techriique Il in purifying the enzyme. For higher enzyme yield, technique II would seem to be a better one than technique I.Item Molecular improvement of food functional properties of Soybean glycinin by protein engineering(Tanzania Journal of Agricultural Sciences) Gidamis, A. B.; Nnko, S. A.; Bunzo, M.; Utsumi, S.; Kito, M.Item The quality of water in small community supplies of Kingolwira village, Morogoro, Tanzania(Tanzania Health Research) Shayo, N. B.; Chove, B. E.; Gidamis, A. B.; Ngoma, O. B.