Browsing by Author "Lamont, Susan"
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Item Antibody response, viral load, viral clearance and growth rate in Tanzanian free-range local chickens infected with lentogenic Newcastle disease virus(Academic Journals) Gallardo, Rodrigo; Kelly, Terra; Lamont, Susan; Dekkers, Jack; Zhou, Huaijun; Muhairwa, Amandus; Mushi, James Richard; Chiwanga, Gaspar Honorati; Mollel, Esther Lemburis; Walugembe, Muhammed; Max, Robert Arsen; Msoffe, Peter Makenga; Rodrigo GallardoItem Antibody response, viral load, viral clearance and growth rate in Tanzanian free-range local chickens infected with lentogenic Newcastle disease virus(Academic Journals, 2021-04-15) Gallardo, Rodrigo; Kelly, Terra; Lamont, Susan; Dekkers, Jack; Zhou, Huaijun; Muhairwa, Amandus; Mushi, James Richard; Chiwanga, Gaspar Honorati; Mollel, Esther Lemburis; Walugembe, Muhammed; Max, Robert Arsen; Msoffe, Peter Makenga; Rodrigo GallardoThis study is aimed at evaluating antibody responses, viral loads, viral clearance and growth rate of Tanzanian free-range local chicken (FRLC) challenged with LaSota strain of Newcastle disease virus (NDV) as indicator traits for selection of chickens for breeding with enhanced resistance to the disease and economic value. Three popular free-range local chicken ecotypes: Kuchi, Ching’wekwe and Morogoro-medium from three ecological zones of Tanzania were used for the experiments. Progenies from the breeder chickens were challenged with 10 7 titer of 50% egg infectious dose (EID 50 ) of the virus at 28 days of age. The viral loads and viral clearance rates evaluated by qRT-PCR from tear samples collected at 2- and 6-days post infection (dpi) showed that Kuchi could clear NDV better than Morogoro- medium and Ching’wekwe. Anti-NDV antibody levels determined from blood samples collected at 10 dpi using ELISA showed that Kuchi ecotype expressed higher mean anti-NDV antibodies compared to Morogoro-medium and Ching’wekwe. Growth rates determined from body weights collected for 38 days from day of hatch (D0) to 10 dpi showed higher growth rate for Kuchi than Morogoro-medium and Ching’wekwe chickens. Kuchi chickens were potentially more resistant to ND compared to Morogoro- medium and Ching’wekwe.Item Conserved, breed-dependent, and subline-dependent innate immune responses of Fayoumi and Leghorn chicken embryos to Newcastle disease virus infection.(Scientific Reports) Schilling, Megan; Memari, Sahar; Cavanaugh, Meredith; Katani, Robab; Deist, Melissa; Radzio-Basu, Jessica; Lamont, Susan; Buza, Joram; Kapur, VivekItem Transcriptional Innate Immune Response of the Developing Chicken Embryo to Newcastle Disease Virus Infection(Frontiers in Genetics) Schilling, Megan; Katani, Robab; Memari, Sahar; Cavanaugh, Meredith; Buza, Joram; Radzio-Basu, Jessica; Mpenda, Fulgence; Deist, Melissa; Lamont, Susan; Kapur, Vivek