Browsing by Author "Mshinda, Hassan"
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Item Drug coverage in treatment of malaria and the consequences for resistance evolution – evidence from the use of sulphadoxine/ pyrimethamine(Malaria Journal) Malisa, Allen L.; Pearce, Richard J.; Abdulla, Salim; Mshinda, Hassan; Kachur, Patrick S.; Bloland, Peter; Roper, CallyItem Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania(Malaria Journal) Mbugi, Erasto V; Mutayoba, Benezeth M; Malisa, Allen L; Balthazary, Sakurani T; Nyambo, Thomas B; Mshinda, HassanItem Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania(Malaria Journal, 2006-10) Mbugi, Erasto V; Mutayoba, Benezeth M; Malisa, Allen L; Balthazary, Sakurani T; Nyambo, Thomas B; Mshinda, HassanBackground: Sulphadoxine-pyrimethamine (SP) has been and is currently used for treatment of uncomplicated Plasmodium falciparum malaria in many African countries. Nevertheless, the response of parasites to SP treatment has shown significant variation between individuals. Methods: The genes for dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) were used as markers, to investigate parasite resistance to SP in 141 children aged less than 5 years. Parasite DNA was extracted by Chelex method from blood samples collected and preserved on filter papers. Subsequently, polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were applied to detect the SP resistance-associated point mutations on dhfr and dhps. Commonly reported point mutations at codons 51, 59, 108 and 164 in the dhfr and codons 437, 540 and 581 in the dhps domains were examined. Results: Children infected with parasites harbouring a range of single to quintuple dhfr/dhps mutations were erratically cured with SP. However, the quintuple dhfr/dhps mutant genotypes were mostly associated with treatment failures. High proportion of SP resistance-associated point mutations was detected in this study but the adequate clinical response (89.4%) observed clinically at day 14 of follow up reflects the role of semi-immunity protection and parasite clearance in the population. Conclusion: In monitoring drug resistance to SP, concurrent studies on possible confounding factors pertaining to development of resistance in falciparum malaria should be considered. The SP resistance potential detected in this study, cautions on its useful therapeutic life as an interim firstline drug against malaria in Tanzania and other malaria-endemic countries.Item Ecologists Can Enable Communities to Implement Malaria Vector Control in Africa(BioMed Central) Mukabana, Richard W.; Kannady, Khadija; Kiama, Michael G.; Ijumba, Jasper N.; Mathenge, Evan M.; Kiche, Ibrahim; Nkwengulila, Gamba; Mboera, Leonard; Mtasiwa, Deo; Yamagata, Yoichi; Schayk, Ingeborg van; Knols, Bart G. J.; Lindsay, Steven W.; Castro, Marcia C.; Mshinda, Hassan; Tanner, Marcel; Fillinger, Ulrike; Killeen, Gerry F.Item Media, health workers, and policy makers’ relationship and their impact on antimalarial policy adoption: a population genetics perspective(Sokoine University of Agriculture, Tanzania.) Malisa, Allen L; Pearce, Richard J; Mutayoba, Benezeth; Abdullah, Salim; Mshinda, Hassan; Kachur, Patrick; Bloland, Peter; Roper, CallyItem Multiplicity of infections and level of recrudescence in Plasmodium falciparum malaria in Mlimba, Tanzania(Academic Journals) Mbugi, Erasto V.; Mutayoba, Benezeth M; Balthazary, Sakurani T; Malisa, Allen L; Nyambo, Thomas B; Mshinda, HassanItem Quantification of markers of antimalarial drug resistance from an area of high malaria transmission: Comparing frequency with prevalence(Academic Journals) Malisa, Allen L.; Pearce, Richard J.; Mutayoba, Ben; Abdullah, Salim; Mshinda, Hassan; Kachur, Patrick; Bloland, Peter; Roper, CallyItem The evolution of pyrimethamine resistant dhfr in Plasmodium falciparum of south-eastern Tanzania: comparing selection under SP alone vs SP +artesunate combination(Malaria Journal) Malisa, Allen L; Pearce, Richard J; Mutayoba, Ben M; Abdullah, Salim; Mshinda, Hassan; Kachur, Patrick S; Bloland, Peter; Roper, CallyItem The genetic change in P. falciparum populations of rural Tanzania resulting from national policy on firstline malaria treatment and pilot Sulfadoxine/pyrimethamine and Artesunate combination(Malaria Journal) Malisa, Allen L; Pearce, Richard J; Abdullah, Salim; Mshinda, Hassan; Kachur, Patrick; Bloland, Peter; Roper, CallyItem Therapeutic efficacy of sulfadoxine-pyrimethamine and prevalence of resistance markers in Tanzania prior to revision of malaria treatment policy: Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase mutations in monitoring in vivo resistance(The American Society of Tropical Medicine and Hygiene) Mugittu, Kefas; Ndejembi, Modesta; Malisa, Allen; Lemnge, Martha; Premji, Zulfikar; Mwita, Alex; Nkya, Watoky; Kataraihya, Johannes; Abdulla, Salim; Beck, Hans-Peter; Mshinda, Hassan