Browsing by Author "Ziwa, Michael H."

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Evidence of Yersinia pestis DNA in rodents in plague outbreak foci in Mbulu and Karatu Districts, northern Tanzania
Ziwa, Michael H.; Matee, Mecky I.; Kilonzo, Bukheti S.; Hang’ombe, Bernard M.
Evidence of Yersinia pestis DNA in rodents in plague outbreak foci in Mbulu and Karatu Districts, northern Tanzania
(2013-07) Ziwa, Michael H.; Matee, Mecky I.; Kilonzo, Bukheti S.; Hang’ombe, Bernard M.
Human plague remains a public health concern in Tanzania despite its quiescence in most foci for years, considering the recurrence nature of the disease. Appreciable researches have involved serological screening of rodents, fleas and humans but none has involved molecular detection and hence proving the presence of Yersinia pestis in rodents in the most recent affected foci, Mbulu and Karatu districts in northern Tanzania. The objective of the current study was to employ a simple PCR to detect Yersinia pestis plasminogen activator (pla) gene in various potential mammalian hosts/reservoirs. The study was conducted in five villages in Mbulu and one in Karatu districts during the period of no disease outbreak. Rodents and small wild carnivores were captured, anaesthetized, identified, sexed and autopsied. Liver, spleen, heart and lung specimens were collected and DNA extracted after which PCR was used to detect the Y. pestis pla gene. A total of 517 small mammals were captured; of which, 493 (95.4%) were from Mbulu and 24 (4.6%) from Karatu. Two Mastomys natalensis (one from each district) and one Gerbilliscus sp. in Mbulu district were positive for Y. pestis pla gene. In conclusion, our results have provided a proof on the presence of Y. pestis in the two rodent species (Mastomys natalensis and Gerbilliscus sp.) and thus providing indicative evidence that the two are potential reservoirs of the pathogen and hence may be responsible for maintaining the same during periods of no disease outbreaks.
Plague in Tanzania: an overview
Ziwa, Michael H.; Matee, Mecky I.; Hang’ombe, Bernard M.; Lyamuya, Eligius F.; Kilonzo, Bukheti S
Plague in Tanzania: an overview
(2013-10) Ziwa, Michael H.; Matee, Mecky I.; Hang’ombe, Bernard M.; Lyamuya, Eligius F.; Kilonzo, Bukheti S
Human plague remains a public health concern in Tanzania despite its quiescence in most foci for years, considering the recurrence nature of the disease. Despite the long-standing history of this problem, there have not been recent reviews of the current knowledge on plague in Tanzania. This work aimed at providing a current overview of plague in Tanzania in terms of its introduction, potential reservoirs, possible causes of plague persistence and repeated outbreaks in the country. Plague is believed to have been introduced to Tanzania from the Middle East through Uganda with the first authentication in 1886. Xenopsylla brasiliensis, X. cheopis, Dinopsyllus lypusus, and Pulex irritans are among potential vectors while Lophuromys spp, Praomys delectorum, Graphiurus murinus, Lemniscomys striatus, Mastomys natalensis, and Rattus rattus may be the potential reservoirs. Plague persistence and repeated outbreaks in Tanzania are likely to be attributable to a complexity of factors including cultural, socio-economical, environmental and biological. Minimizing or preventing people’s proximity to rodents is probably the most effective means of preventing plague outbreaks in humans in the future. In conclusion, much has been done on plague diagnosis in Tanzania. However, in order to achieve new insights into the features of plague epidemiology in the country, and to reorganize an effective control strategy, we recommend broader studies that will include the ecology of the pathogen, vectors and potential hosts, identifying the reservoirs, dynamics of infection and landscape ecology