| dc.creator |
Mrema, John George |
|
| dc.date |
2015-03-12T05:36:10Z |
|
| dc.date |
2015-03-12T05:36:10Z |
|
| dc.date |
2011-06 |
|
| dc.date.accessioned |
2019-12-06T12:04:07Z |
|
| dc.date.available |
2019-12-06T12:04:07Z |
|
| dc.identifier |
http://hdl.handle.net/123456789/134 |
|
| dc.identifier.uri |
http://hdl.handle.net/123456789/14829 |
|
| dc.description |
This report has been prepared by John G. Mrema a candidate for the program of MSc. Clinical Research and supervised by Dr. Reginald Kavishe. This research report is submitted as requirements for the Degree of Master of Science in Clinical Research of Tumaini University.
Background: Despite reports on declining malaria prevalence, malaria has remained a major public health problem. One of the several strategies to control malaria is blocking of the transmission cycle through drugs or a vaccine. Previously, it has been reported that single dose primaquine is capable of reducing days of gametocyte circulation after malaria treatment but safety in individuals with Glucose-6-Phosphate dehydrogenase (G6PD) deficiency is still a challenging question. Recently, the use of single dose primaquine showed significant reduction in haemoglobin (Hb) level in both G6PD deficiency and non-G6PD deficiency individuals. This might be contributed by other genetic factors including Glutathione S-Transferase (GST) deficiencies.
Justification: Primaquine is an effective drug against gametocyte stage of Plasmodium. The hemolytic effect of primaquine needs to be addressed properly for proper use of the drug in malaria control. This study investigated the relationship between primaquine-associated hemolysis and GSTT1.
Research question: Do glutathione S-Transferases theta-1 polymorphism influence the hemoglobin status of children after administration of single dose primaquine in addition to SP and atersunate?
Aim: The aim of this study was to assess the role of GSTT1 genotyic polymorphisms on the Hb status among children who received single dose of primaquine in addition to SP-artesunate in lower Moshi.
Specific objectives: The study was specifically determine the prevalence of GSTT1genotype among the children in Lower Moshi and the association between GSTT1genotypic polymorphism with Hb status of children after receiving single dose primaquine in addition to SP + artesunate.
Research Methodology: This was a retrospective cross-sectional study conducted in Kilimanjaro region at Lower Moshi. Whereby 242 DNA samples collected from children aged from 1 to 12 years after receiving single dose primaquine in addition to SP + artesunate were genotyped using multiplex PCR.
Results: Wild type GSTT1 was significantly associated with Hb reduction (p=0.03). Considering G6PD, we observe highly significant association with percentage Hb decrease (p=0.0000) among G6PD deficient participants. We also observe highly statistical significance among children with both G6PD deficiency and wild type GSTT1 (p=0.0000). Conclusions: Hb reduction after single dose of primaquine in addition to SP-AS in children may be contributed by other genetic factors such as GSTT1 but G6PD deficiency remains to be the major factor. Further studies to explore the role of other genetic factors are necessary for the rational use of primaquine in control of malaria transmission. |
|
| dc.language |
en |
|
| dc.subject |
Research Subject Categories::MEDICINE |
|
| dc.title |
THE INFLUENCE OF GLUTATHIONE S-TRANSFERASE THETA-1 GENOTYPIC POLYMORPHISM ON HEMOGLOBIN STATUS OF CHILDREN AFTER ADMINISTRATION OF SINGLE DOSE PRIMAQUINE |
|
| dc.type |
Thesis |
|