| dc.creator |
Kajungiro, Redempta A |
|
| dc.creator |
Xue, Liang |
|
| dc.creator |
Mehary, Aynealem |
|
| dc.date |
2015-12-17T08:29:06Z |
|
| dc.date |
2015-12-17T08:29:06Z |
|
| dc.date |
2015-01 |
|
| dc.date.accessioned |
2018-03-27T08:22:01Z |
|
| dc.date.available |
2018-03-27T08:22:01Z |
|
| dc.identifier |
Kajungiro, R.A., Xue, L., Aynealem, M. (2015) Molecular Cloning and Expression Patterns of Two Tumor Necrosis Factor Alpha Genes in Crucian carp (Carassius carassius). Molecular Biology, 49(1): 120-129 pp. |
|
| dc.identifier |
0026-8933 |
|
| dc.identifier |
http://hdl.handle.net/123456789/147 |
|
| dc.identifier.uri |
http://hdl.handle.net/123456789/2250 |
|
| dc.description |
Tumor necrosis factor (TNF[31]) is a potent inflammatory cytokine produced during inflammation. In this study, two crucian carp TNF-α genes, TNFα-1 and TNFα-2, were cloned and sequenced. The TNFα-1 is 720 bp long and consists of a 699 bp opening reading frame (ORF) encoding 232 amino acids and TNFα-2 is 793 bp long and contains an ORF of 687 bp, which encodes 228 amino acids. The genomic structure of both genes consists of 4 exons and 3 introns similar to the other known TNF-α genes. The amino acid sequences of the crucian carp TNFα-1 and TNFα-2 share the highest identity with common carp, goldfish and the lowest with flounder and humans. The phylogenetic analysis grouped crucian carp TNFα-1 and TNFα-2 with other cypriniformes, which are more closely related to goldfish and common carp TNFα-1 and TNFα-2. Real time PCR analysis showed a constitutive expression of crucian carp TNFα-1 and TNFα-2 in all seven tissues examined. The TNFα-1 mRNA was expressed significantly higher in the liver and kidney than those of TNFα-2, while TNFα-2 in turn was expressed significantly higher in the muscle. The Aeromonas hydrophila BSK-10 strain upregulated the expression level of both TNFα-1 and TNFα-2 in all of the tested tissues. At 6 h, the expression levels of TNFα-1 were increased significantly higher in the muscles, skin, and liver, while the expression levels of TNFα-2 were increased significantly higher in the muscles and gills. TNFα-1 was expressed much stronger than TNFα-2. At 12 h the expression levels started to decline and were even lower at 24 h. These results imply that both TNFα-1 and TNFα-2 mRNA are distributed differently in tissues and are implicated in the immune response to bacterial infection. |
|
| dc.language |
en |
|
| dc.publisher |
Pleiades Publishing |
|
| dc.subject |
Aeromonas hydrophila BSK-10 |
|
| dc.subject |
Crucian carp Carassius carassius |
|
| dc.subject |
mRNA |
|
| dc.subject |
expression patterns |
|
| dc.subject |
real time PCR |
|
| dc.subject |
tumor necrosis factor α |
|
| dc.title |
Molecular cloning and expression patterns of two tumor necrosis factor alpha genes in crucian carp (Carassius carassius) |
|
| dc.type |
Journal Article |
|