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Molecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia

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dc.creator Nyirenda, S. S.
dc.creator Hang’ombe, B. M.
dc.creator Kilonzo, B. S.
dc.creator Kabeta, M. N.
dc.creator Cornellius, M.
dc.creator Sinkala, Y.
dc.date 2018-06-20T06:12:32Z
dc.date 2018-06-20T06:12:32Z
dc.date 2016
dc.date.accessioned 2019-12-20T12:36:29Z
dc.date.available 2019-12-20T12:36:29Z
dc.identifier http://www.suaire.suanet.ac.tz:8080/xmlui/handle/123456789/2415
dc.identifier.uri http://hdl.handle.net/123456789/30900
dc.description Tropical Doctor
dc.description Plague is a re-emerging zoonotic disease caused by the bacterium Yersinia pestis. The disease has caused periodic global devastation since the first outbreak in the 6th century.Twomonths after a suspected plague outbreak inNyimba district, sampleswere collected from94livestock(goats andpigs), 25 rodents, 6 shrews and33 fleas. Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) techniques were used to investigate the presence of Y. pestis, which showed that 16.0% (4/25) of rodents, 16.7% (1/6) of shrews (Crocidura spp) and 6.0% (5/83) of goats were positive for IgG antibodies against Fraction 1 antigen of Y. pestis. Plasminogen activator (Pla) gene (DNA) of Y. pestis was detected in five pools containing 36.4% (12/33) fleas collected from pigs (n¼4), goats (n¼5) and rodents (n¼3). The detection of Pla gene in fleas and IgG antibodies against Fraction1 antigen in rodents, shrews and goats suggest that Y. pestis had been present in the study area in the recent past.
dc.language en
dc.publisher Sage
dc.subject Domestic pigs
dc.subject Fleas
dc.subject Goats
dc.subject Plague
dc.subject Rodents
dc.subject Zambia
dc.title Molecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia
dc.type Article


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