Delimitation of Termitomyces species principally rely on the macro-micromorphological characteristics of the species although recently they are supplemented by molecular techniques. Utility of these characters have never been examined to authenticate their usefulness. The present paper therefore, provides a detailed study on the efficacy of the macro and micro-morphological characters used in classifying Termitomyces species. Macromorphologically, capsize was very useful and based on it, three groups were established. Other more useful characters include colour of the cap and pseudorrhiza presence. Annulus presence, pseudorrhiza colour, size, and morphometry were apparently unuseful. Micromorphology was less informative and ambiguously used while untetra basidiospore character was revealed for the first time in this genus. In general macromorphological characteristics provide more reliable taxonomic information to delineate most of the species in the genus than micromophology. However, for the more similar species, an analysis of more stable molecular characters is required. More distinguishing characters are yet to be revealed while ascertaining the used one and taxonomic status of T. citriophylus is essential.