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Isolation and Identification of Local Ethanolic Yeasts Inhabiting Coffee Processing Environments in Tanzania

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dc.creator Hamadi, Said
dc.creator Muruke, Masoud H.
dc.creator Hosea, Ken M.M.
dc.date 2015-11-19T12:50:13Z
dc.date 2015-11-19T12:50:13Z
dc.date 2014-12-20
dc.date.accessioned 2021-05-03T13:36:16Z
dc.date.available 2021-05-03T13:36:16Z
dc.identifier Hamadi, S., Muruke, M. H and Hosea, K.M.M (2014) Isolation and identification of local ethanolic yeasts inhabiting coffee processing environments in Tanzania, International Journal of Life Sciences, 3.(4) : 213-221
dc.identifier http://hdl.handle.net/123456789/120
dc.identifier.uri http://hdl.handle.net/123456789/47574
dc.description Coffee processing environment harbours different microbiota of fungi and bacteria, some of which are of great economic value. This study isolated, screened and identified indigenous yeast associated with ethanol production potential in coffee processing environments of Mbinga in Ruvuma region and Hai in Kilimanjaro region using standard procedures. Yeast identification was done by amplification and sequencing D1/D2 domain of the gene 26S rDNA. A total of 21 yeast isolates were obtained from both sites and only 8 of them were able to ferment glucose. All 9 isolates fermented coffee pulp waste to ethanol at varying levels. Highest ethanol production was observed by isolate M4, which produced 3% (v/v) ethanol, followed by PDA4MB (2.8% v/v), PDA3 (2.6% v/v) and the least was by M2 which produced only 1.1% (v/v).Three isolates showed good attribute to ethanol production (2.76 to 3.56% v/v) even after an addition of higher sugar concentrations to coffee pulp waste. The basic local alignment search tool (BLAST) and phylogenetic analysis revealed the identity of the yeasts as Pichia kudriavzevii, Issatchenkia orientalis, Clavispora lusitaniae, Pichia guilliermondii, and Pichia anomala, all of them known to be associated with ethanol fermentation. Their attributes towards ethanol fermentation are taken to be potential for further investigation for bioethanol production.
dc.description World Bank project C1A 3.3 “Industrial bioconversion of selected Tanzanian crops and residues into value added products using biotechnology”
dc.language en
dc.subject Coffee pulp waste
dc.subject Isolation
dc.subject Ethanol
dc.subject Yeast
dc.subject Sequencing
dc.title Isolation and Identification of Local Ethanolic Yeasts Inhabiting Coffee Processing Environments in Tanzania
dc.type Journal Article, Peer Reviewed


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