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Molecular cloning and expression patterns of two tumor necrosis factor alpha genes in crucian carp (Carassius carassius)

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dc.creator Kajungiro, Redempta A
dc.creator Xue, Liang
dc.creator Mehary, Aynealem
dc.date 2015-12-17T08:29:06Z
dc.date 2015-12-17T08:29:06Z
dc.date 2015-01
dc.date.accessioned 2021-05-03T13:36:17Z
dc.date.available 2021-05-03T13:36:17Z
dc.identifier Kajungiro, R.A., Xue, L., Aynealem, M. (2015) Molecular Cloning and Expression Patterns of Two Tumor Necrosis Factor Alpha Genes in Crucian carp (Carassius carassius). Molecular Biology, 49(1): 120-129 pp.
dc.identifier 0026-8933
dc.identifier http://hdl.handle.net/123456789/147
dc.identifier.uri http://hdl.handle.net/123456789/47601
dc.description Tumor necrosis factor (TNF[31]) is a potent inflammatory cytokine produced during inflammation. In this study, two crucian carp TNF-α genes, TNFα-1 and TNFα-2, were cloned and sequenced. The TNFα-1 is 720 bp long and consists of a 699 bp opening reading frame (ORF) encoding 232 amino acids and TNFα-2 is 793 bp long and contains an ORF of 687 bp, which encodes 228 amino acids. The genomic structure of both genes consists of 4 exons and 3 introns similar to the other known TNF-α genes. The amino acid sequences of the crucian carp TNFα-1 and TNFα-2 share the highest identity with common carp, goldfish and the lowest with flounder and humans. The phylogenetic analysis grouped crucian carp TNFα-1 and TNFα-2 with other cypriniformes, which are more closely related to goldfish and common carp TNFα-1 and TNFα-2. Real time PCR analysis showed a constitutive expression of crucian carp TNFα-1 and TNFα-2 in all seven tissues examined. The TNFα-1 mRNA was expressed significantly higher in the liver and kidney than those of TNFα-2, while TNFα-2 in turn was expressed significantly higher in the muscle. The Aeromonas hydrophila BSK-10 strain upregulated the expression level of both TNFα-1 and TNFα-2 in all of the tested tissues. At 6 h, the expression levels of TNFα-1 were increased significantly higher in the muscles, skin, and liver, while the expression levels of TNFα-2 were increased significantly higher in the muscles and gills. TNFα-1 was expressed much stronger than TNFα-2. At 12 h the expression levels started to decline and were even lower at 24 h. These results imply that both TNFα-1 and TNFα-2 mRNA are distributed differently in tissues and are implicated in the immune response to bacterial infection.
dc.language en
dc.publisher Pleiades Publishing
dc.subject Aeromonas hydrophila BSK-10
dc.subject Crucian carp Carassius carassius
dc.subject mRNA
dc.subject expression patterns
dc.subject real time PCR
dc.subject tumor necrosis factor α
dc.title Molecular cloning and expression patterns of two tumor necrosis factor alpha genes in crucian carp (Carassius carassius)
dc.type Journal Article


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