dc.creator |
Kilonzo, B. S. |
|
dc.creator |
Madoffe, S. S. |
|
dc.creator |
Maliondo, S. M. S. |
|
dc.creator |
Msanya, B. M. |
|
dc.creator |
Mabagalla, R. |
|
dc.creator |
Kitojo, D. H. |
|
dc.date |
2016-12-20T05:22:31Z |
|
dc.date |
2016-12-20T05:22:31Z |
|
dc.date |
2001 |
|
dc.date.accessioned |
2022-10-25T08:50:40Z |
|
dc.date.available |
2022-10-25T08:50:40Z |
|
dc.identifier |
https://www.suaire.sua.ac.tz/handle/123456789/1125 |
|
dc.identifier.uri |
http://hdl.handle.net/123456789/90482 |
|
dc.description |
The article is a result of a project titled: The Impact
and fate of pesticidal proteins from Bacillus
thuringiensis spp. in tropical soils and waters as
influenced by interactions with clays - MICROBE
- (Project No. INCO no ERBICI8 CT 970135) |
|
dc.description |
Laboratory colonies of Anopheles gambiae and Culex quinquefasciatus were reared from parental larvae
obtained from the Ifakara Health Research Centre and septic tanks ill Morogoro Municipality
respectively. Water ponds (1M3
) were constructed at selected sites and left open for mosquitoes to breed
ill freely. Various concentrations of Bti crystals were-mixed with varying concentrations of clay and tested
against third instar larvae of each species at various periods of time post-preparation. A total of 60 larvae
(20 x 3) were exposed to each mixture in the laboratory, and each- experiment was replicated four times.
Mortality rates were recorded after 241t. exposure.
The observations revealed that for every concentration of Bti crystals used, the toxicity on both species of
mosquitoes was less persistent in crystal/clay mixtures than in crystals alone. It was also shown that such
persistence was much less in mixtures containing large concentrations of clay (5mg/ml) titan in those
containing low concentrations (0.05 - 0.5 mg/ml) of clay. It wasfurther observed th at the toxicity against
Culex and Anopheles larvae was more persistent in mixtures containing higher concentrations of Bti
crystals (0.12 - 0.3 ug/ml) titan ill those containing low concentrations (0.03 - 0.06 ug/ml) of the toxin.
Furthermore, it was observed that C. quinquefasciatus larvae succumbed to Bti crystal/clay mixtures for
longer periods titan A. gambiae larvae.
It was generally concluded that clays have 11 negative impact on the persistence of Bti toxicity against
mosquito larvae and th at such impact is more significant in mixtures containing large concentrations
clay. It was also concluded that despite the environmental friendliness of Bti, its applicability by
communities is limited in view of its short persistence when it is adsorbed to tropical soils which are found
in most natural mosquito breeding sites. |
|
dc.description |
European
Union (EU) |
|
dc.format |
application/pdf |
|
dc.language |
en |
|
dc.subject |
BTi Toxicity |
|
dc.subject |
Mosquito larvae |
|
dc.subject |
C. quinquefasciatus larvae |
|
dc.subject |
A. gambiae larvae |
|
dc.title |
The effect of clay on the persistence of BTi toxicity against mosquito larvae in Morogoro, Tanzania IA |
|
dc.type |
Article |
|