Existence of multiple ESBL genes among phenotypically conﬁrmed ESBL producing klebsiella pneumoniae and escherichia coli concurrently isolated from clinical, colonization and contamination samples from neonatal units at Bugando Medical Center, Mwanza, Tanzania

Silago, Vitus; Kovacs, Dory; Samson, Happyness; Seni, Jeremiah; Matthews, Louise; Oravcová, Katarina; Lupindu, Athumani M.; Hoza, Abubakar S.; Mshana, Stephen E.

dc.creator	Silago, Vitus
dc.creator	Kovacs, Dory
dc.creator	Samson, Happyness
dc.creator	Seni, Jeremiah
dc.creator	Matthews, Louise
dc.creator	Oravcová, Katarina
dc.creator	Lupindu, Athumani M.
dc.creator	Hoza, Abubakar S.
dc.creator	Mshana, Stephen E.
dc.date	2021-04-29T09:09:11Z
dc.date	2021-04-29T09:09:11Z
dc.date	2021
dc.date.accessioned	2022-10-25T08:50:43Z
dc.date.available	2022-10-25T08:50:43Z
dc.identifier	Silago, V.; Kovacs, D.; Samson, H.; Seni, J.; Matthews, L.; Oravcová, K.; Lupindu, A.M.; Hoza, A.S.; Mshana, S.E. Existence of Multiple ESBL Genes among Phenotypically Conﬁrmed ESBL Producing Klebsiella pneumoniae and Escherichia coli Concurrently Isolated from Clinical, Colonization and Contamination Samples from Neonatal Units at Bugando Medical Center, Mwanza, Tanzania. Antibiotics 2021, 10, 476. https://doi.org/ 10.3390/antibiotics10050476
dc.identifier	https://www.suaire.sua.ac.tz/handle/123456789/3497
dc.identifier.uri	http://hdl.handle.net/123456789/90567
dc.description	Journal article
dc.description	The proportions and similarities of extended-spectrum β-lactamase (ESBL) producing K. pneumoniae (ESBL-KP) and E. coli (ESBL-EC) carrying multiple ESBL genes is poorly known at our setting. This study investigated the existence of multiple ESBL genes (blaCTX-M, blaTEM, and blaSHV) among ESBL-KP and ESBL-EC concurrently isolated from clinical, colonization, and contamination samples from neonatology units in Mwanza-Tanzania. Twenty and 55 presumptive ESBL-EC and ESBL-KP, respectively, from a previous study archived at−80 ◦C were successfully recovered for this study. Isolates were screened and conﬁrmed for production of ESBLs by phenotypic methods followed by multiplex PCR assay to determine ESBL genes. All (100%) and 97.3% of presumptive ESBL isolates were phenotypically conﬁrmed by Clinical and Laboratory Standards Institute (CLSI) and modiﬁed double-disc synergy methods, respectively. About 93.3% (70/75) of phenotypically conﬁrmed ESBL isolates had at least one ESBL gene, whereby for 62.9% (44/70), all ESBL genes (blaCTX-M, blaTEM, and blaSHV) were detected. Eight pairs of ESBL bacteria show similar patterns of antibiotics susceptibility and ESBL genes. ESBL-KP and ESBL-EC, concurrently isolated from clinical, colonization and contamination samples, harbored multiple ESBL genes. Further, eight pairs of ESBL isolates had similar patterns of antibiotics susceptibility and ESBL genes, suggesting transmission of and/or sharing of mobile genetic elements (MGEs) among ESBL-KP and ESBL-EC.
dc.format	application/pdf
dc.language	en
dc.publisher	MDPI
dc.subject	Antimicrobial resistance
dc.subject	Extended-spectrum beta-lactamase
dc.subject	Escherichia coli
dc.subject	Klebsiella pneumoniae
dc.title	Existence of multiple ESBL genes among phenotypically conﬁrmed ESBL producing klebsiella pneumoniae and escherichia coli concurrently isolated from clinical, colonization and contamination samples from neonatal units at Bugando Medical Center, Mwanza, Tanzania
dc.type	Article