International journal of science: basic and applied research, 2018; 38 (1): 70-81Heat shock response is rapidly induced to protect cells from irreversible injury by stabilizing cellular synthetic and metabolic activities. Particularly, the response differentially protects against stresses, infections and inflammations. While it could be time- and tissue-dependent, it is not established as to how it exhibits. This study explored the expression of heat shock protein (Hsp) 27, 70 and 90 in intestines, kidneys, livers and lungs of mice undergoing hyperthermia at 42°C for 1 hr and recovering for 1-72 hr. The expression of these Hsps was determined by immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections. There was an increase in the expression of Hsp27, 70 and 90 that peaked at 6-12 hr and differentially declined at 48-72 hr. The peak expression was reached earlier in the lung and dropped sharply in the intestine while sustained for a longer time in the liver and kidney. At 72 hr only Hsp90 exhibited moderate expression in the lung and kidney. It is concluded that optimal expression of Hsps is time- and tissue- dependent and has narrow margin of peak expression in some tissues. This knowledge might contribute to designing therapeutic agents for curbing stresses, infections and inflammations that can be suppressed by Hsps.