| dc.creator |
Gidamis, A.B. |
|
| dc.creator |
Nnko, S.A. |
|
| dc.creator |
Shayo, N.B. |
|
| dc.creator |
Chove, B.E. |
|
| dc.creator |
Kroner, H. K. |
|
| dc.date |
2017-12-08T11:27:35Z |
|
| dc.date |
2017-12-08T11:27:35Z |
|
| dc.date |
1998 |
|
| dc.date.accessioned |
2022-10-25T08:50:59Z |
|
| dc.date.available |
2022-10-25T08:50:59Z |
|
| dc.identifier |
https://www.suaire.sua.ac.tz/handle/123456789/1845 |
|
| dc.identifier.uri |
http://hdl.handle.net/123456789/90896 |
|
| dc.description |
Tanzania Journal of Agriculture Science 1998, Vol. 1(2), pp 141-148 |
|
| dc.description |
A stlldy was conducted to compare the efficiency of two methods of microbial cell separation
and enzyme purification using penicillin-G-acylase (PGA) from Escherichia coli. The efficiency
of two methods of ceJ[ separation; centrifugation and cross flow filtration (CFF) were
compared. The CFF method was found to have both higher separation efficiency and enzyme
yield than centrifugation method. Centrifugation method gave a separation efficiency of
98.5% with enzyme yield of94 % whereas CFFmethod resulted in 100% separation efficiency
and enzyme yield of98.8%. The Escherichia coli cells were disrupted by high pressure homogenization
(HPH),and the disrupted cells were purified using two different techniques.
Technique I was a combination of cross-flow-diafiltration (CFD) , ultrafiltration (UF) and
heat/pH-shift treatments. This technique resulted in 47% enzyme yield with a purification factor
of 12. Technique II which involved two extraction steps by' aqueous two - phase system
(APS) coupled with UF resulted in 62 % enzyme yield with a pu;ificationfactor of 4. Technique
I was therefore much better than techriique Il in purifying the enzyme. For higher enzyme
yield, technique II would seem to be a better one than technique I. |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.subject |
Microbial enzymes |
|
| dc.subject |
Purification factor |
|
| dc.subject |
Recovery rate |
|
| dc.subject |
Separation efficiency |
|
| dc.subject |
Technical enzyme |
|
| dc.title |
Comparative evaluation of different methods of extraction and purification used in technical enzyme production from microorganisms |
|
| dc.type |
Article |
|