Molecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia

Nyirenda, S. S.; Hang’ombe, B. M.; Kilonzo, B. S.; Kabeta, M. N.; Cornellius, M.; Sinkala, Y.

dc.creator	Nyirenda, S. S.
dc.creator	Hang’ombe, B. M.
dc.creator	Kilonzo, B. S.
dc.creator	Kabeta, M. N.
dc.creator	Cornellius, M.
dc.creator	Sinkala, Y.
dc.date	2018-06-20T06:12:32Z
dc.date	2018-06-20T06:12:32Z
dc.date	2016
dc.date.accessioned	2022-10-25T08:51:07Z
dc.date.available	2022-10-25T08:51:07Z
dc.identifier	https://www.suaire.sua.ac.tz/handle/123456789/2415
dc.identifier.uri	http://hdl.handle.net/123456789/91048
dc.description	Tropical Doctor
dc.description	Plague is a re-emerging zoonotic disease caused by the bacterium Yersinia pestis. The disease has caused periodic global devastation since the first outbreak in the 6th century.Twomonths after a suspected plague outbreak inNyimba district, sampleswere collected from94livestock(goats andpigs), 25 rodents, 6 shrews and33 fleas. Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) techniques were used to investigate the presence of Y. pestis, which showed that 16.0% (4/25) of rodents, 16.7% (1/6) of shrews (Crocidura spp) and 6.0% (5/83) of goats were positive for IgG antibodies against Fraction 1 antigen of Y. pestis. Plasminogen activator (Pla) gene (DNA) of Y. pestis was detected in five pools containing 36.4% (12/33) fleas collected from pigs (n¼4), goats (n¼5) and rodents (n¼3). The detection of Pla gene in fleas and IgG antibodies against Fraction1 antigen in rodents, shrews and goats suggest that Y. pestis had been present in the study area in the recent past.
dc.format	application/pdf
dc.language	en
dc.publisher	Sage
dc.subject	Domestic pigs
dc.subject	Fleas
dc.subject	Goats
dc.subject	Plague
dc.subject	Rodents
dc.subject	Zambia
dc.title	Molecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia
dc.type	Article