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The chemical constituents of Toussantia orientalis and T. patriciae (Annonaceae) leaf extracts were
evaluated for their antiviral activities in ovo against Infectious Bursal Disease Virus (IBDV) and Newcastle
Disease Virus (NDV). The nine-day-old embryonated eggs in a set of five were used for testing through the
allantoic route inoculation assay for each tested compound and controls. For NDV assay, the allantoic fluids
from the specimens were further harvested to determine viral contagion. The tested compounds exhibited
potency with varying levels of significance at a screening concentration of 360 μg/ml against the two viral
strains. Embryos infected with IBDV survived, grew to normal size with complete organ formation and had
mean weights comparable to those of the uninfected ones when treated with the aminocinnamoyl tetraketides 1,
2, and 4, glucosylflavonoid 7 from T. orientalis and ursolic acid derivatives 9 and 10 from T. patriciae
demonstrating high efficacy against IBDV. The compounds also exhibited antiviral activity against NDV,
showing viral titre reduction ranging from 1:16 - 1:256 in the haemagglutination test, with compound 11
having the lowest titre value (1:16) followed by compound 7 (1:32). The compounds that exhibited significant
antiviral efficacy could be considered potential leads for the development of antiviral agents. |
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