The ring-tailed lemur (Lemur catta) is primarily distributed in south and south-western Madagascar. It is classified as an endangered species by the International Union for Conservation of Nature and Natural Resources. The ring-tailed lemur is a species commonly kept in zoological gardens. Diseases involving various body systems such as musculoskeletal, digestive, circulatory, respiratory and urogenital systems have been reported in the ring-tailed lemur, which threatens their survival. This review describes the normal anatomy of the ring-tailed lemur as a reference for clinical use and species identification. Variations exist in the normal anatomy of different animal species. Knowledge of the normal anatomy of individual species is important for accurate diagnosis and treatment of diseases.