Multiplicity of infections and level of recrudescence in Plasmodium falciparum malaria in Mlimba, Tanzania

Mbugi, Erasto V.; Mutayoba, Benezeth M; Balthazary, Sakurani T; Malisa, Allen L; Nyambo, Thomas B; Mshinda, Hassan

dc.creator	Mbugi, Erasto V.
dc.creator	Mutayoba, Benezeth M
dc.creator	Balthazary, Sakurani T
dc.creator	Malisa, Allen L
dc.creator	Nyambo, Thomas B
dc.creator	Mshinda, Hassan
dc.date	2016-11-30T10:45:45Z
dc.date	2016-11-30T10:45:45Z
dc.date	2006-09
dc.date.accessioned	2022-10-25T08:53:06Z
dc.date.available	2022-10-25T08:53:06Z
dc.identifier	1684–5315
dc.identifier	https://www.suaire.sua.ac.tz/handle/123456789/1035
dc.identifier.uri	http://hdl.handle.net/123456789/93351
dc.description	African Journal of Biotechnology Vol. 5 (18), pp. 1655-1662, 18 September 2006, © 2006 Academic Journals
dc.description	Polymorphism and antigenic variation are important biological survival strategies of malaria parasites determining the episode, outcome and implications of treatment interventions. In P. falciparum, polymorphic antigens are associated with the asexual blood-stage; merozoite surface protein 2 (MSP2). The MSP2 genes have been invaluable in post-treatment discrimination of parasite resurgence from new infection, especially in high transmission areas. We performed polymerase chain reaction (PCR) on DNA extracted from blood samples of 141 malaria-infected infants, followed by restriction fragment length polymorphism (RFLP) of PCR products. The findings showed multiplicity of infections of single to six infections with an average of 2.58 infections per patient. Single infections of either 3D7 or FC27 allelic families of the MSP2 gene occurred in 51 patients (50.5%) out of all PCR-RFLP successful samples (n = 101). Out of 15 (10.6%) follow up samples with resurgent parasitaemia, 3 (20%) samples had recrudescent infections while 12 (80%) had variable results. Our findings provide an insight on the prevalence of the genetic determinants of suphadoxine-pyrimethamine (SP) resistance in Mlimba during the study period, and in the face of rapidly spreading resistance, calls for the periodic surveillance in order to timely detect early warning signal of the deteriorating SP cure rate.
dc.format	application/pdf
dc.language	en
dc.publisher	Academic Journals
dc.subject	Multiplicity infections
dc.subject	recrudescence-Plasmodium
dc.subject	Plasmodium falciparum malaria
dc.subject	Malaria parasites
dc.subject	MSP2 genes
dc.subject	Mlimba
dc.subject	Tanzania
dc.title	Multiplicity of infections and level of recrudescence in Plasmodium falciparum malaria in Mlimba, Tanzania
dc.type	Article