| dc.creator |
Misinzo, G. |
|
| dc.creator |
Delputte, P. L. |
|
| dc.creator |
Lefebvre, D. J. |
|
| dc.creator |
Nauwynck, H. J. |
|
| dc.date |
2017-06-23T06:28:22Z |
|
| dc.date |
2017-06-23T06:28:22Z |
|
| dc.date |
2007-12-17 |
|
| dc.date.accessioned |
2022-10-25T08:53:06Z |
|
| dc.date.available |
2022-10-25T08:53:06Z |
|
| dc.identifier |
https://www.suaire.sua.ac.tz/handle/123456789/1671 |
|
| dc.identifier.uri |
http://hdl.handle.net/123456789/93358 |
|
| dc.description |
Archives virology 2008, Vol. 153: 337–342 |
|
| dc.description |
Treatment of porcine kidney (PK-15) cells with
either interferon-gamma (IFN-g) or endosomallysosomal
system acidification inhibitors increases
replication of porcine circovirus type 2 (PCV2). In
the present study, the effect of a combination of
these treatments on the number of infected cells
and virus yield was tested. The number of PCV2
(Stoon-1010)-infected PK-15 cells increased in
cells treated with ammonium chloride (445 39%
increase), IFN-g (446 8%), ammonium chlorideþ
IFN-g (1721 283%), chloroquine diphosphate
(1037 121%), chloroquine diphosphateþIFN-g
(2199 255%), monensin (950 178%) and
monensinþIFN-g (1948 60%). Combined IFNg
and endosomal-lysosomal system acidification
inhibitors increased PCV2 yield by up to 50 times
compared to untreated PK-15. This augmented virus
replication in PK-15 cells may be helpful in the
production of PCV2 vaccines. |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.subject |
Porcine circovirus-2 |
|
| dc.subject |
PK-15 cells |
|
| dc.subject |
Endosomallysosomal system |
|
| dc.subject |
Interferon-gamma (IFN-g) |
|
| dc.title |
Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomallysosomal system acidification |
|
| dc.type |
Article |
|