MICROSATELLITE LETTERSThe Black and Rufous sengi, Ryhnchocyon petersi, is endemic to a limited range in East Africa. We report the development of eight polymorphic microsatellites using next generation sequencing technology. Eighteen individuals from Zaraninge forest (Saadani National Park, Tanzania) were genotyped. The number of alleles per locus ranged from 2 to 6, while the observed and expected heterozygosities varied from 0.17 to 0.82 and from 0.25 to 0.81, respectively. No locus deviated from Hardy–Weinberg equilibrium. These microsatellite markers will be useful tools to study the effect of habitat fragmentation on the population genetic structure of R. petersi.