Urinary tract infections (UTI) during pregnancy can lead to serious obstetric complications, poor maternal and perinatal outcomes. The purpose of this study was to determinethe prevalence of bacterial UTI in pregnant women in Morogoro municipality, Tanzania. This study was conducted at SabaSaba Health Centre from May 2014 to July 2014 involving 196 pregnant women. Mid stream of urine samples were obtained and analyzed by dipstick and processed for culture. Out of 196 pregnant women studied 13.8% had significant bacterial UTI. Escherichia coli were the most prevalent isolated bacteria with 12 isolates (44.4%).Other isolated organisms were Klebsiella species (3; 11.1%), Proteus species (2; 7.4%), Enterococcus faecalis (3; 11.1%), Coagulase negative Staphylococcus (5; 19%) and Staphylococcus aureus (2; 7.1%).Antimicrobial sensitivity test results showed that out of12 E. coli isolates, 33%, 42%, 50%, 50%, 67%, 83%, were resistant to ciprofloxacin, ampicillin, tetracycline, amoxicillin, cotrimoxazole and erythromycin respectively. Out of three Klebsiella species, 33% were resistant to ciprofloxacin, 67% were resistant to ampicillin, amoxicillin and tetracycline and 100% were resistant to cotrimoxazole and erythromycin. Two Proteus species isolated showed 100% resistance to tetracycline and cotrimoxazole and 0% resistance to amoxicillin, ampicillin, ciprofloxacin and erythromycin. Two Staphylococci aureus isolates showed 50% resistance to ampicillin, amoxicillin, tetracycline, ciprofloxacin, cotrimoxazole and erythromycin. Five Coagulase negative Staphylococci isolates showed 20% resistance to ampicillin, amoxicillin and ciprofloxacin, 60% to tetracycline, cotrimoxazole and erythromycin.Three Entrerococcus faecalis isolates showed 100% resistance to ampicillin, amoxicillin, tetracycline, ciprofloxacin, ii cotrimoxazole and erythromycin. Using univariate analysis there was no significant association of age, parity, gestational age, education, marital status, and history of UTI with bacteriuria. The study showed that only ciprofloxacin could be the drug of choice for treatment UTI, but it is contraindicated during pregnancy. Further studies of antimicrobial sensitivity of isolates fromUTI during pregnancy are recommended.