This research article was published in the Journal of Hospitality Management and Tourism Vol. 13(2), July-December 2022This review paper assesses the role of tour operators towards sustaining ecotourism in Tanzania. The motive behind it is based on the argument that tour operators have an important role within the ecotourism multi-stakeholder. However, little has been documented on how best to engage them for sustainable ecotourism. Thus, this paper provides a concise overview of the relevant literature, highlighting existing knowledge and gaps for prospective future research. In addition to the literature review and methods, the result section of the paper is organized in such a way that it elaborates on key concepts and provides the setup and organization of tour operators. As a core part of the review, this study underlined four key roles of tour operators towards sustainable ecotourism: their involvement in planning, development, promotion, and implementation of ecotourism products. The study concluded that ecotourism supporting and regulating machinery must work closely and strategically with tour operators at the individual level or through their existing tourism associations in order to ensure that those four identified areas are effectively and efficiently carried out, not only to enhance their business but also to conserve and market the destination sites and contribute to the country’s revenue.