A Dissertation Submitted in Partial Fulfilment of the Requirements for the Degree of Master of Science in Public Health Research of the Nelson Mandela African Institution of Science and TechnologyThis study explored utility of cone bioassays for pre-delivery quality assurance (QA) of pyrethroid insecticide-treated nets (ITNs) to test the assumption that cone bioassays are consistent across locations, mosquito strains, and laboratories. Double-blinded bioassays were conducted on 20 pyrethroid ITNs of four brands (100 nets, 5 subsamples per net) that had been delivered for mass distribution in Papua New Guinea (PNG) having passed pre-delivery inspections. Cone bioassays were performed on the same net pieces following World Health Organization (WHO) guidelines at the PNG Institute of Medical Research (PNGIMR) using pyrethroid susceptible Anopheles farauti sensu stricto and at Ifakara Health Institute (IHI), Tanzania using pyrethroid susceptible Anopheles gambiae sensu stricto. Results from IHI and PNGIMR were compared using Spearman’s Rank correlation, Bland-Altman (BA) analysis and analysis of agreement. In cone bioassays, 13/20 nets (65%) at IHI and 8/20 (40%) at PNGIMR met WHO bio-efficacy criteria. Results from IHI and PNGIMR correlated on 60-minute knockdown (KD60) (rs= 0.6, p= 0.002, n=20) and 24-hour mortality (M24) (rs=0.9, p<0.0001, n=20) but BA showed systematic bias between the results. The agreement between the results to predict ITN failure was good with kappa=0.79 (0.53-1.00) and 90% accuracy. Based on these study findings, the WHO cone bioassay is a reproducible bioassay for ITNs with >80% M24, and for all ITNs provided inherent stochastic variation and systematic bias are accounted for. The 80% mortality (M24) threshold remains the most reliable indicator of pyrethroid ITN quality using pyrethroid susceptible mosquitoes. In the absence of alternative tests, cone bioassays could be used as part of pre-delivery QA.