| dc.creator |
Lyantagaye, Sylvester L. |
|
| dc.date |
2016-05-05T14:10:44Z |
|
| dc.date |
2016-05-05T14:10:44Z |
|
| dc.date |
2014-12 |
|
| dc.date.accessioned |
2018-03-27T08:59:56Z |
|
| dc.date.available |
2018-03-27T08:59:56Z |
|
| dc.identifier |
Lyantagaye, S.L., 2014. Characterization of the Biochemical Pathway of Apoptosis Induced by D-glucopyranoside Derivatives from Tulbaghia violacea. Annual Research & Review in Biology, 4(6), p.962. |
|
| dc.identifier |
http://hdl.handle.net/20.500.11810/1838 |
|
| dc.identifier.uri |
http://hdl.handle.net/20.500.11810/1838 |
|
| dc.description |
Aim: Chinese hamster ovary (CHO) cells were used to identify the most likely apoptotic
biochemical pathway induced by Methyl-α-D-glucopyranoside (MDG), D-fructofuranose- β(2→6)-methyl-α-D-glucopyranoside (DFMDG) and β-D-fructofuranosyl-(2→6)-α-D- glucopyranoside (DFDG) treatments isolated from wild garlic (Tulbaghia violacea).
Methodology: Assays, which detect different morphological and biochemical changes in
the CHO cells were used to examine the apoptosis induction by individual agents, paying
special attention to the mitochondria membrane potential (∆ψm) depolarization.
Results: The major events of apoptosis, namely, translocation of phosphatidylserine,
mitochondria membrane potential (∆ψm) depolarization and activation of caspase-3 in
CHO cells were initiated within 2 hours of treatments. The onset of the apoptosis induction
was indistinguishable among the MDG, DFMDG and DFDG, and similar to that of
staurosporine.
Conclusion: The study shows that CHO cells treated with MDG, DFMDG and DFDG
undergo apoptotic death through the mitochondrial (intrinsic) pathway. |
|
| dc.language |
en |
|
| dc.subject |
Apoptosis |
|
| dc.subject |
Mitochondrial pathway |
|
| dc.subject |
Glucopyranosides |
|
| dc.subject |
Mitochondrial permeability transition pore |
|
| dc.title |
Characterization of the Biochemical Pathway of Apoptosis Induced by Three Dglucopyranoside Derivatives from Tulbaghia Violacea |
|
| dc.type |
Other |
|