| dc.creator |
Kidibule, Peter |
|
| dc.creator |
Sosovele, Eva M. |
|
| dc.creator |
Mshandete, Anthony M. |
|
| dc.date |
2016-05-17T13:28:32Z |
|
| dc.date |
2016-05-17T13:28:32Z |
|
| dc.date |
2014 |
|
| dc.date.accessioned |
2018-03-27T09:00:16Z |
|
| dc.date.available |
2018-03-27T09:00:16Z |
|
| dc.identifier |
Kidibule, P.E., Sosovele, E.M. and Mshandete, A.M., 2014. Isolation and Identification of Microorganisms from Crude oil Contaminated Soils of Dar es Salaam, Tanzania. British Biotechnology Journal, 4(8), p.918. |
|
| dc.identifier |
http://hdl.handle.net/20.500.11810/2091 |
|
| dc.identifier |
10.9734/BBJ/2014/11780 |
|
| dc.identifier.uri |
http://hdl.handle.net/20.500.11810/2091 |
|
| dc.description |
Aims: The aim of this study was to isolate and identify microorganisms in crude oil polluted
soils in Eastern Tanzania.
Study Design: Isolation and identification of microorganisms using conventional and
molecular techniques.
Place and Duration of Study: Department of Molecular Biology & Biotechnology,
University of Dar es Salaam; October 2012 to June 2013.
Methodology: Soils were collected from Mlalakua (ML), University of Dar es Salaam
(UDSM) and Ubungo (Ub) local garages in Dar es Salaam, Tanzania. The selective
isolation of microorganisms was done based on colony morphological appearance on agar
plates and Gram reaction. Three isolates (one from each site) were subjected to molecular
methods for the identification purposes. Phylogenetic analyses of bacterial 16S rRNA gene
and fungal ITS region sequences were employed in this study to identify and determine the
evolutionary relationships of the ML, Ub and UDSM isolates.
Results: The 16S rRNA analysis revealed that the ML isolate belonged to the genus
Klebsiella, while the ITS analysis on Ub and UDSM isolates assigned them to the genus
Trichosporon and Candida, respectively.
Conclusions: From these findings, further studies are recommended to assay for the
biodegradative potentiality of the identified isolates for bioremediation of crude oil
contaminated soils. |
|
| dc.language |
en |
|
| dc.subject |
Isolation |
|
| dc.subject |
Identification |
|
| dc.subject |
Microorganisms |
|
| dc.subject |
Crude oil |
|
| dc.subject |
Soils |
|
| dc.title |
Isolation and Identification of Microorganisms from Crude oil Contaminated Soils of Dar es Salaam, Tanzania |
|
| dc.type |
Journal Article, Peer Reviewed |
|