| dc.identifier |
Patrick, F., Mtui, G., Mshandete, A.M. and Kivaisi, A., 2010. Optimized Production of Lignin Peroxidase, Manganese Peroxidase and Laccase in Submerged Cultures of Trametes Trogii Using Various Growth Media Compositions. Tanzania Journal of Science, 36(1). |
|
| dc.description |
A white-rot fungus, Trametes trogii, was isolated from coastal Tanzania and screened for crude
lignolytic enzymes production using Rhemazol Brilliant blue R (RBBR) dye, 2,2-azino-bis (3-
ethylbenzthiazoline)-6-sulfonate (ABTS) and guaiacol in a semi-solid medium. Lignin peroxidase
(LiP), manganese peroxidase (MnP) and Laccase (Lac) were detected by pyrogallol and !-
napthol solutions, respectively on the guaiacol supplemented solid media. The effect of
temperature, pH, carbon, nitrogen, Cu2+, 2,5-xylidine, ferulic acid, varatryl alcohol and Mn2+ in
submerged culture fermentations were investigated for maximum enzymes production. After 7
days of incubation, 72-100% oxidation of RBBR, ABTS and guaiacol was observed. With
optimized culture conditions, the fungal filtrate had maximum LiP, MnP and Lac activities of
0.18, 4.44 and 593 U/ml, respectively compared to 0.0011, 0.0054 and 2.3 U/ml obtained with
non-optimized ones, amounting to 16,264%, 82,122% and 25,683% increase in LiP, MnP and
Lac activities, respectively. The enhanced crude enzymes activities, RBBR decolorization and
ABTS guaiacol oxidation capabilities of T. trogii show its potential as a source of industrial
enzymes for biotechnological applications. |
|