| dc.creator |
Patrick, Ferdinandi |
|
| dc.creator |
Mtui, Godliving |
|
| dc.creator |
Mshandete, Anthony M. |
|
| dc.creator |
Johansson, Gunnar |
|
| dc.creator |
Kivaisi, Amelia K. |
|
| dc.date |
2016-05-17T13:31:23Z |
|
| dc.date |
2016-05-17T13:31:23Z |
|
| dc.date |
2009 |
|
| dc.date.accessioned |
2018-03-27T09:00:30Z |
|
| dc.date.available |
2018-03-27T09:00:30Z |
|
| dc.identifier |
Patrick, F., Mtui, G., Mshandete, A.M., Johansson, G. and Kivaisi, A., 2009. Purification and characterization of a laccase from the basidiomycete Funalia trogii (Berk.) isolated in Tanzania. Afr. J. Biochem. Res, 3(5), pp.250-258. |
|
| dc.identifier |
1996-0778 |
|
| dc.identifier |
http://hdl.handle.net/20.500.11810/2112 |
|
| dc.identifier.uri |
http://hdl.handle.net/20.500.11810/2112 |
|
| dc.description |
A lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal
Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete
rhemazol brilliant blue - R (RBBR) decolorization 2,2’-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and
guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593
U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion
chromatography to good purity as judged by sodium dodecyl sulfate-polyacrylamide gel
electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). The isolated main component had a
molecular weight of ca 58 kDa as determined by MS and an isoelectric point (pI) of 3.8. The optimal pH
and temperature range for the purified laccase were 4.0 - 5.0 and 50 - 70 ºC, respectively, using 2, 6-
dimethoxyphenol as a substrate. |
|
| dc.language |
en |
|
| dc.subject |
Funalia trogii |
|
| dc.subject |
Laccase |
|
| dc.subject |
Anion exchange chromatography |
|
| dc.subject |
Size exclusion chromatography |
|
| dc.subject |
Electrophoresis |
|
| dc.subject |
Isoelectric focusing |
|
| dc.title |
Purification and Characterization of a Laccase from the Basidiomycete Funalia Trogii (Berk.) Isolated in Tanzania |
|
| dc.type |
Journal Article, Peer Reviewed |
|