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High Temperature Simultaneous Saccharification and Fermentation of Starch from Inedible Wild Cassava (Manihot Glaziovii) to Bioethanol Using Caloramator Boliviensis

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dc.creator Moshi, Anselm P.
dc.creator Hosea, Ken M.
dc.creator Elisante, Emrode
dc.creator Mamo, G.
dc.creator Mattiasson, Bo
dc.date 2016-06-26T16:58:33Z
dc.date 2016-06-26T16:58:33Z
dc.date 2015
dc.date.accessioned 2018-03-27T09:00:35Z
dc.date.available 2018-03-27T09:00:35Z
dc.identifier Moshi, A.P., Hosea, K.M., Elisante, E., Mamo, G. and Mattiasson, B., 2015. High temperature simultaneous saccharification and fermentation of starch from inedible wild cassava (Manihot glaziovii) to bioethanol using Caloramator boliviensis. Bioresource technology, 180, pp.128-136.
dc.identifier http://hdl.handle.net/20.500.11810/2666
dc.identifier 10.1016/j.biortech.2014.12.087
dc.identifier.uri http://hdl.handle.net/20.500.11810/2666
dc.description Full text can be accessed at http://www.sciencedirect.com/science/article/pii/S0960852414018549
dc.description The thermoanaerobe, Caloramator boliviensis was used to ferment starch hydrolysate from inedible wild cassava to ethanol at 60 °C. A raw starch degrading α-amylase was used to hydrolyse the cassava starch. During fermentation, the organism released CO2 and H2 gases, and Gas Endeavour System was successfully used for monitoring and recording formation of these gaseous products. The bioethanol produced in stoichiometric amounts to CO2 was registered online in Gas Endeavour software and correlated strongly (R2 = 0.99) with values measured by HPLC. The organism was sensitive to cyanide that exists in cassava flour. However, after acclimatisation, it was able to grow and ferment cassava starch hydrolysate containing up to 0.2 ppm cyanide. The reactor hydrogen partial pressure had influence on the bioethanol production. In fed-batch fermentation by maintaining the hydrogen partial pressure around 590 Pa, the organism was able to ferment up to 76 g/L glucose and produced 33 g/L ethanol.
dc.language en
dc.publisher Elsevier
dc.subject Bioenergy
dc.subject Manihot spp.
dc.subject Thermoanaerobes
dc.subject Gas Endeavour System
dc.subject Caloramator boliviensis
dc.title High Temperature Simultaneous Saccharification and Fermentation of Starch from Inedible Wild Cassava (Manihot Glaziovii) to Bioethanol Using Caloramator Boliviensis
dc.type Journal Article


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