| dc.creator |
Akhmanova, Anna |
|
| dc.creator |
Voncken, Frank |
|
| dc.creator |
Harhangi, Harry R. |
|
| dc.creator |
Hosea, Ken M. |
|
| dc.creator |
Vogels, Godfried D. |
|
| dc.creator |
Hackstein, Johannes H. P. |
|
| dc.date |
2016-06-26T17:11:00Z |
|
| dc.date |
2016-06-26T17:11:00Z |
|
| dc.date |
1998 |
|
| dc.date.accessioned |
2018-03-27T09:00:38Z |
|
| dc.date.available |
2018-03-27T09:00:38Z |
|
| dc.identifier |
Akhmanova, A., Voncken, F.G., Harhangi, H., Hosea, K.M., Vogels, G.D. and Hackstein, J.H., 1998. Cytosolic enzymes with a mitochondrial ancestry from the anaerobic chytrid Piromyces sp. E2. Molecular microbiology, 30(5), pp.1017-1027. |
|
| dc.identifier |
http://hdl.handle.net/20.500.11810/2689 |
|
| dc.identifier |
10.1046/j.1365-2958.1998.01130.x |
|
| dc.identifier.uri |
http://hdl.handle.net/20.500.11810/2689 |
|
| dc.description |
The anaerobic chytrid Piromyces sp. E2 lacks mitochondria,
but contains hydrogen-producing organelles,
the hydrogenosomes. We are interested in how
the adaptation to anaerobiosis influenced enzyme compartmentalization
in this organism. Random sequencing
of a cDNA library from Piromyces sp. E2 resulted
in the isolation of cDNAs encoding malate dehydrogenase,
aconitase and acetohydroxyacid reductoisomerase.
Phylogenetic analysis of the deduced amino
acid sequences revealed that they are closely related
to their mitochondrial homologues from aerobic
eukaryotes. However, the deduced sequences lack
N-terminal extensions, which function as mitochondrial
leader sequences in the corresponding mitochondrial
enzymes from aerobic eukaryotes. Subcellular
fractionation and enzyme assays confirmed that the
corresponding enzymes are located in the cytosol.
As anaerobic chytrids evolved from aerobic, mitochondria-bearing
ancestors, we suggest that, in the
course of the adaptation from an aerobic to an anaerobic
lifestyle, mitochondrial enzymes were retargeted
to the cytosol with the concomitant loss of their
N-terminal leader sequences. |
|
| dc.language |
en |
|
| dc.title |
Cytosolic Enzymes with a Mitochondrial Ancestry from the Anaerobic Chytrid Piromyces Sp. E2. Mol Microbiol 30:1017-1027 |
|
| dc.type |
Journal Article, Peer Reviewed |
|