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Production of Lactic Acid from Waste Sisal Stems Using a Lactobacillus Isolate

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dc.creator Muruke, M. H. S.
dc.creator Hosea, Ken M.
dc.creator Pallangyo, A.
dc.creator Heijthuijsen, J. H. F. G.
dc.date 2016-06-26T17:43:36Z
dc.date 2016-06-26T17:43:36Z
dc.date 2006
dc.date.accessioned 2018-03-27T09:00:38Z
dc.date.available 2018-03-27T09:00:38Z
dc.identifier Muruke, M.H.S., Hosea, K.M., Pallangyo, A. and Heijthuijsen, J.H.F.G., 2006. Production of lactic acid from waste sisal stems using lactobacillus isolate. Discovery and Innovation, 18(1), pp.5-10.
dc.identifier http://hdl.handle.net/20.500.11810/2775
dc.identifier.uri http://hdl.handle.net/20.500.11810/2775
dc.description Suitability of waste sisal stems as substrate for industrial production of lactic acid was studied. Local bacteria were isolated and screened for growth and lactic acid production from inulin, a polymeric carbohydrate present in the sisal stems. Four bacterial isolates were found to grow and produce lactic acid from sisal stem inulin. The best isolate, coded LAB4 produced 49g/L lactic acid from 150g/L sisal stem medium after 96 hours fermentation at optimal conditions (pH 6.5 and a temperature of 42°C). Furthermore, lactic acid production increased by 33% when calcium carbonate (5% final concentration) was included in the medium. Supplementation with yeast extract (0.05 - 0.2% final concentration) did not significantly affect lactic acid production. Preliminary morphological and physiological studies indicate that strain LAB4 belongs to the genus Lactobacillus. From these initial results, we propose both the bacterial isolate LAB4 and the waste sisal stems for further studies on industrial scale lactic acid production.
dc.language en
dc.title Production of Lactic Acid from Waste Sisal Stems Using a Lactobacillus Isolate
dc.type Journal Article


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