Carbohydrate Analysis of Pulps Using Enzymatic Hydrolysis and High Pressure Liquid Chromato- Graphy
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A revised method for analysis of carbohydrates in
pulps is presented. The method utilizes commercially
available enzyme preparations for enzymatic hydrolysis
of the pulp, followed by acid hydrolysis for complete
cleavage of oligomers that are still present.
Quantification of monosaccharides in the hydrolysate
was performed by HPLC using a Pb-sulphonate/polymer
column and deionized water as the mobile phase.
Integration of the chromatograms was performed using
least squares fitting of partially resolved peaks by commercial
software. The method requires moderate operator
skills and low investment costs for specialized
analytical equipment.