| dc.creator |
Mbuna, Julius |
|
| dc.creator |
Kaneta, Takashi |
|
| dc.date |
2016-07-14T20:46:52Z |
|
| dc.date |
2016-07-14T20:46:52Z |
|
| dc.date |
2014-12 |
|
| dc.date.accessioned |
2018-03-27T08:54:52Z |
|
| dc.date.available |
2018-03-27T08:54:52Z |
|
| dc.identifier |
Mbuna, J. and Kaneta, T., 2015. Capillary Electrophoresis with Laser-induced Fluorescence Detection for Application in Intracellular Investigation of Anthracyclines and Multidrug Resistance Proteins. Analytical Sciences, 31(11), pp.1121-1128. |
|
| dc.identifier |
http://hdl.handle.net/20.500.11810/3189 |
|
| dc.identifier |
DOI: 10.2116/analsci.31.1121 |
|
| dc.identifier.uri |
http://hdl.handle.net/20.500.11810/3189 |
|
| dc.description |
Capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) is a powerful method for the trace analysis of cellular components. This review presents a summary of topics for the direct analysis of anthracyclines and multidrug resistance proteins in cancerous cells. A micellar electrokinetic chromatography (MEKC) method that does not use organic solvents, and hence prevents the precipitation of proteins in cellular samples, was shown to be a reliable method for the determination of several anthracyclines including the epimeric doxorubicin and epirubicin. A fast CE-based immunoassay for investigating transporter multidrug resistance associated protein (MRP1) was also developed for routine or explorative analysis of the levels of transporter proteins in cancerous cells. A combination of the developed MEKC-LIF method and the CE immunoassay (CEIA) method has permitted the analysis of anthracyclines and MRP1 in a cell line to show the relationship between the levels of MRP1 and amount of anthracyclines in cancerous cells. |
|
| dc.language |
en |
|
| dc.subject |
Capillary electrophoresis |
|
| dc.subject |
Micellar electrokinetic chromatography |
|
| dc.subject |
Capillary electrophoresis immunoassay |
|
| dc.subject |
Laser-induced fluorescence |
|
| dc.subject |
Anthracycline |
|
| dc.subject |
Multidrug resistance associated protein |
|
| dc.subject |
Cancer cell |
|
| dc.title |
Capillary Electrophoresis with Laser-induced Fluorescence Detection for Application in Intracellular Investigation of Anthracyclines and Multidrug Resistance Proteins |
|
| dc.type |
Journal Article, Peer Reviewed |
|