| dc.creator |
Kurosumi, Akihiro |
|
| dc.creator |
Kobayasi, Fumihisa |
|
| dc.creator |
Mtui, Godliving Y. S. |
|
| dc.creator |
Nakamura, Yoshitoshi |
|
| dc.date |
2016-09-21T14:21:45Z |
|
| dc.date |
2016-09-21T14:21:45Z |
|
| dc.date |
2006-05 |
|
| dc.date.accessioned |
2018-03-27T09:00:43Z |
|
| dc.date.available |
2018-03-27T09:00:43Z |
|
| dc.identifier |
Kurosumi, A., Kobayasi, F., Mtui, G. and Nakamura, Y., 2006. Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent. Biochemical engineering journal, 30(1), pp.109-113. |
|
| dc.identifier |
http://hdl.handle.net/20.500.11810/4030 |
|
| dc.identifier.uri |
http://hdl.handle.net/20.500.11810/4030 |
|
| dc.description |
The optimal culture condition of basidiomycete Sparassis crispa mycelia was determined for producing a large amount of antineoplastic constituent, i.e. β-1,3-glucan. Furthermore, a new extraction method with steam explosion as a pretreatment was attempted to increase the amount of antineoplastic constituent extracted from S. crispa mycelia. The shake-flask liquid culture of fungal mycelia could shorten a culture time up to about 1/3 compared with the conventional stationary liquid culture. The productivity of S. crispa mycelia reached its maximum at an initial glucose concentration of 30 g/L, a culture temperature of 25–30 °C, and pH 5. Steam explosion at a steam temperature of 225 °C for 5 min not only enhanced the amount of antineoplastic constituent extracted from the mycelia but also shortened the extraction time significantly. |
|
| dc.language |
en |
|
| dc.subject |
Bioconversion |
|
| dc.subject |
Filamentous fungi |
|
| dc.subject |
β-1,3-Glucan |
|
| dc.subject |
Microbial growth |
|
| dc.subject |
Plant cell culture |
|
| dc.subject |
Sparassis crispa |
|
| dc.title |
Development of optimal Culture Method of Sparassis crispa mycelia and new extraction method of Antineoplastic Constituent. |
|
| dc.type |
Journal Article, Peer Reviewed |
|