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Cytotoxic and antimalarial biflavonoids isolated from the aerial parts of Ochna serrulata (Hochst.) Walp

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dc.creator Ndoile, Monica
dc.creator Van Heerden, Fanie
dc.date 2018-09-10T09:56:10Z
dc.date 2018-09-10T09:56:10Z
dc.date 2018
dc.date.accessioned 2021-05-03T13:19:55Z
dc.date.available 2021-05-03T13:19:55Z
dc.identifier Ndoile, M., & van Heerden, F. (2018). Cytotoxic and antimalarial biflavonoids isolated from the aerial parts of Ochna serrulata (Hochst.) Walp. Tanzania Journal of Science, 44(3), 152-162.
dc.identifier http://hdl.handle.net/20.500.11810/4854
dc.identifier.uri http://hdl.handle.net/20.500.11810/4854
dc.description This paper reports the phytochemistry and biological activities (antimalarial and cytotoxicity) of compounds isolated from aerial parts of Ochna serrulata. Silica gel chromatography followed by repeated chromatotron on the stem bark extract of the plant yielded a new dimeric chalcone (5-deoxyurundeuvine C) along with lophirone C, lophirone A, afzelone B, epicatechin, methyl (2′,4′-dihydroxyphenyl)acetate and a mixture of campylospermone A and isocampylospermone A. From the leaves extract, ochnaflavone, 2″,3″-dihydroochnaflavone, vitexin-6″-O-acetate, 3′-methoxyvitexin-6″-O-acetate and (2′,4′-dihydroxyphenyl)acetic acid were isolated and characterized. A cyanoglucoside was isolated from an extract of flowers and fruits of the plant. The proposed structures of the respective new and known compounds were assigned by using 1D and 2D NMR techniques as well as Mass spectrometry. The cytotoxicity activity of the compounds was tested using sulforhodamine B (SRB) assay in three different cancer cell lines namely, UACC62 (melanoma), TK 10 (renal), and MCF7 (breast). Ochnaflavone and 3′-methoxyvitexin-6″-O-acetate inhibited the growth of UACC62 at TGI (Total Growth Inhibition concentration) 12.91 and 14.11 µM, respectively. The cytotoxic activities of lophirone C were found to be TGI = 35.63, 11.67 and 30.35 µM, against TK, UACC62 and MCF7 cancer cells respectively. Lophirone A exhibited TGI against these cancer cells at 58.96, 26.23 and 40.01 µM, respectively. The rest of the compounds exhibited no significant cytotoxicity against the three cancer cells. Moreover, the compounds were evaluated for antimalarial activity against FCR-3 (chloroquine-resistant Plasmodium falciparum) strains. Ochnaflavone demonstrated the highest activity IC50 = 17.25 µM, followed by lophirone A (29.78 µM), 5-deoxyurundeuvine C (31.07 µM), lophirone C (35.31 µM), afzelone B (39.54 µM), 2″,3″-dihydroochnaflavone (61.86 µM) and 3′-methoxyvitexin-6″-O-acetate (106.35 µM). These results concur with the traditional use of the genus in the treatment of various ailments.
dc.language en
dc.subject 5-Deoxyurundeuvine C
dc.subject Biflavonoid
dc.subject Cytotoxicity
dc.subject Antimalarial
dc.subject Ochna serrulata
dc.title Cytotoxic and antimalarial biflavonoids isolated from the aerial parts of Ochna serrulata (Hochst.) Walp
dc.type Journal Article, Peer Reviewed


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