| dc.creator |
Rwegasila, Edward |
|
| dc.creator |
Munissi, Joan J.E |
|
| dc.creator |
Mubofu, Egid B. |
|
| dc.creator |
Nyandoro, Stephen S. |
|
| dc.creator |
Erasto, Paul |
|
| dc.date |
2018-11-08T10:09:52Z |
|
| dc.date |
2018-11-08T10:09:52Z |
|
| dc.date |
2018-06 |
|
| dc.date.accessioned |
2021-05-03T13:19:56Z |
|
| dc.date.available |
2021-05-03T13:19:56Z |
|
| dc.identifier |
Edward Rwegasila, Joan J.E. Munissi, Egid B. Mubofu, Stephen S. Nyandoro and Paul Erasto, In vivo antimycobacterial studies of toussaintine A-chitosan nanocomposites, Tanzania Journal of Science. 2018, 44 (2), 16 - 26. |
|
| dc.identifier |
0856-1761 |
|
| dc.identifier |
http://hdl.handle.net/20.500.11810/4965 |
|
| dc.identifier.uri |
http://hdl.handle.net/20.500.11810/4965 |
|
| dc.description |
Chitosan (CS, molecular weight (MW) 20.2 kDA, stability of 210 °C and degree of deacetylation (DD) 73.31%) was obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the studied bioactive natural product, toussaintine A (TA) isolated from the leaves of Toussaintia orientalis, on a chitosan-tripolyphosphate (CS/TPP) nanoformulation was attained through ionotropic gelation. Characterization of pure CS, CS/TPP and TA-CS/TPP nanocomposites was carried out by FTIR and SEM. The encapsulation efficiency and loading capacity of the TA were 69.33 and 0.46%, respectively. The in vitro release kinetics established an initial release of 27% of TA in the initial six hours followed by a slow and maintained release up to 72 h. The in vivo antimycobacterial acitivities of both TA and TA-CS/TPP nanocomposites against Mycobacterium indicus pranii (MIP) employing Galleria mellonella larvae as an infection model were evaluated. TA-CS/TPP nanocomposite formulations exhibited remarkable effectiveness against MIP than free TA |
|
| dc.description |
International Foundation for Science (IFS) |
|
| dc.language |
en |
|
| dc.publisher |
Dar es Salaam University Press |
|
| dc.subject |
Toussaintine A; chitosan; nanocomposites, antimycobacterial; Galleria mellonella |
|
| dc.title |
In vivo antimycobacterial studies of toussaintine A-chitosan nanocomposites |
|
| dc.type |
Journal Article |
|