An attempt has been made to develop anti-microbial textiles by studying the Inhibition zone using honey and turmeric. The concentration of honey and turmeric was as 10:0, 7:3, 5:5, 3:7, and 0:10 and it was applied on to the fabric using direct padding method. The antimicrobial activity of honey and turmeric treated fabric were tested against the infectious bacteria Staphylococcus aureus. Both the samples show better results against the bacteria but honey shows the best results by largest Inhibition Zone diameter.