Full text article. Also available at http://www.saujs.sakarya.edu.tr/en/pub/issue/44246/582641With Mobile IPv6 (MIPv6) protocol support, mobile hosts can move from their home networks or one network to another without interrupting ongoing session. In MIPv6 network, packets from CN to MN undergo rectangular routing owing to long delay and tunneling overhead that affect the performance of mobile network. Furthermore, during handover process packet loss due to MN mobility is high which eventually hinder the performance of real application such as video conference, VoIP. Route optimization methods are applied in mobile network to avoid rectangular routing thus reducing delay, tunneling overhead and improving packet delivery. This paper investigated Return Routability Procedure (RRP) as a route optimization method for MIPv6 networks in terms of packets received, tunneling overhead, route optimization overhead and traffic control received. The result showed that video conference received is the same as if no route optimization was applied. However, comparing tunneled control traffics reduced with route optimization control traffic introduced is negligible. Instead, 0.02% of the tunnel and route optimization overheads were more introduced compared to tunneled and route optimization overheads introduced when no route optimization was applied.