Dissertation (MSc Public Health)Globally, motivation of workers has variables based on high wages, financial reimbursements; non-monetary assistance, promotions, job training and development, and challenging tasks that are accountable for the overall motivation of employees. This dissertation aimed to assess the Direct Health Facility Financing programme on the determinants of healthcare workers’ motivation in the Kigoma Region. A retrospective pre-post design was used coupled with both qualitative and quantitative approaches. The study involved 188 healthcare workers from 43 primary health facilities. An interviewer-administered questionnaire was designed and conducted on 188 healthcare workers and in-depth interviews were done with 30 healthcare workers who had enough knowledge on the operations Direct Health Facility Financing. The determinants of motivation and Direct Health Facility Financing programme were measured through Likert scale ratings prior to and post Direct Health Facility Financing. Quantitative data analysis was done by using the Statistical Package for Social Sciences (SPSS) 26.0 version. Qualitative data were analyzed using the thematic analysis method whereby the recorded data were transcribed and coded to generate themes that helped to answer the research questions. A paired t-test and multiple linear regression model were performed. Healthcare workers in Kigoma are dominated by youth 31.4%, and a majority of them are females (60.6%). Further, a majority of healthcare workers are Certificate holders (61.7%) while Diploma holders are 35.1% of all healthcare workers. The analysis of the data obtained shows that the difference in commitment prior to and post the programme is statistically significant (p=< 0.001). Thus, there was improved overall job satisfaction post-Direct Health Facility Financing (P=0.004). The level of burnout has decreased post-Direct Health Facility Financing. The decrease is statistically significant (P = 0.0320). Health workers perceive decreased workload post Direct Health Facility Financing. Further generalized linear regression results indicated that demographic information and years in employment were not predictors for determinants of health care workers motivation in Kigoma region. Direct Health Facility Financing has positively affected job commitment, job satisfaction, and burnout of healthcare workers in the Kigoma Region.