Dissertation (MSc Public Health)Screening of Gender Based Violence (GBV) is a crucial point to identifying the survivors of GBV and provides the appropriate management for their healing. GBV screening rate among healthcare providers is reported to be low despite the attempts by the Ministry of Health to initiate GBV screening in Tanzania through implementation fidelity. This study attempted to fill the gap by determining the levels of GBV screening in the implementation fidelity, factors affecting the adherence of the implementation fidelity of GBV screening; together with the challenges affecting adherence of the implementation fidelity of GBV screening among the healthcare providers in the health facilities of Dodoma Region. This was an analytical cross-sectional study design; used mixed methods approach. A multi-stage sampling technique was applied to obtain 384 healthcare Providers. The Interviewer-administered questionnaire was used to collect quantitative data. On the other hand, qualitative data were generated through focused group discussions with 21 healthcare providers. SPSS was used for Quantitative analysis of Descriptive statistic to determine the level of adherence, Logistic regression for association of the factors and thematic analysis for challenges affecting adherence to GBV screening. There is a low level of adherence and quality whereby 16.4% and 26.8 % of healthcare providers adhered and had quality implementation fidelity of GBV screening practices respectively. Those who were not trained were less likely to do adherence to the implementation fidelity of GBV screening practices compared to those who were trained (AOR = 0.206, [0.107-0.398] P<0.0001). Nurses were less likely to adhere to GBV screening in reference to the doctors (AOR=0.46, [0.222-0.954], p=0.037). Challenges obtained according to themes were in the part of Managerial and Community issues. The results show that most of the healthcare providers in Dodoma Region do not adhere and perform quality GBV screening. There is a need to address factors and challenge to improve adherence and quality of the implementation fidelity of GBV screening.