Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains
| dc.creator | Rweyemamu, M. M | |
| dc.creator | Booth, J. C | |
| dc.creator | Head, Morwen | |
| dc.creator | Pay, T. W. F | |
| dc.date | 2022-05-17T11:35:39Z | |
| dc.date | 2022-05-17T11:35:39Z | |
| dc.date | 1977-12 | |
| dc.date.accessioned | 2022-10-25T08:51:19Z | |
| dc.date.available | 2022-10-25T08:51:19Z | |
| dc.description | A microneutralization test for serotyping of FMD viruses is described. It is based on earlier observations by Booth, Rweyemamu & Pay (1978) that dose-response relationships in quantal microneutralizations often deviated from linearity. The typing test described therefore utilizes undiluted virus preparations. In about 90 % of samples a positive typing was obtained in contrast with about 50 % for the complement fixation test. The test was also found to be susceptible to minimal quantities of heterotypic viral contamination. For strain differentiation the microneutralization test was carried out as a checkerboard test. When compared with the complement fixation test it was found to be more specific. The necessity to utilize virus-neutralization test systems for comparing FMD virus strains particularly for the purpose of vaccine selection is emphasized. The two dimensional microneutralization test has been applied to a study of comparing FMDV vaccine strains for Europe, South America, the Middle East and East Africa. | |
| dc.format | application/pdf | |
| dc.identifier | https://www.suaire.sua.ac.tz/handle/123456789/4149 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/91240 | |
| dc.language | en | |
| dc.subject | Microneutralization | |
| dc.subject | Serological typing | |
| dc.subject | Mouth disease virus | |
| dc.subject | Subtyping | |
| dc.title | Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains | |
| dc.type | Article |