Comparison of antibiotic resistant Escherichia coli obtained from drinking water sources in northern Tanzania: a cross-sectional study

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dc.creatorLyimo, Beatus
dc.creatorBuza, Joram
dc.creatorSubbiah, Murugan
dc.creatorSmith, Woutrina
dc.creatorCall, Douglas
dc.date2019-10-09T10:34:07Z
dc.date2019-10-09T10:34:07Z
dc.date2016
dc.date.accessioned2022-10-25T09:20:52Z
dc.date.available2022-10-25T09:20:52Z
dc.descriptionResearch Article published by BMC Microbiology
dc.descriptionBackground: Antimicrobial resistance (AMR) is a growing and significant threat to public health on a global scale. Escherichia coli comprises Gram-negative, fecal-borne pathogenic and commensal bacteria that are frequently associated with antibiotic resistance. AMR E. coli can be ingested via food, water and direct contact with fecal contamination. Methods: We estimated the prevalence of AMR Escherichia coli from select drinking water sources in northern Tanzania. Water samples (n = 155) were collected and plated onto Hi-Crome E. coli and MacConkey agar. Presumptive E. coli were confirmed by using a uidA PCR assay. Antibiotic susceptibility breakpoint assays were used to determine the resistance patterns of each isolate for 10 antibiotics. Isolates were also characterized by select PCR genotyping and macro-restriction digest assays. Results: E. coli was isolated from 71 % of the water samples, and of the 1819 E. coli tested, 46.9 % were resistant to one or more antibiotics. Resistance to ampicillin, streptomycin, sulfamethoxazole, tetracycline, and trimethoprim was significantly higher (15–30 %) compared to other tested antibiotics (0–6 %; P < 0.05). Of the β-lactam-resistant isolates, blaTEM-1 was predominant (67 %) followed by blaCTX-M (17.7 %) and blaSHV-1 (6.0 %). Among the tetracyclineresistant isolates, tet(A) was predominant (57.4 %) followed by tet(B) (24.0 %). E. coli isolates obtained from these water sources were genetically diverse with few matching macro-restriction digest patterns. Conclusion: Water supplies in northern Tanzania may be a source of AMR E. coli for people and animals. Further studies are needed to identify the source of these contaminants and devise effective intervention strategies.
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dc.identifierDOI 10.1186/s12866-016-0870-9
dc.identifierhttp://dspace.nm-aist.ac.tz/handle/123456789/473
dc.identifier.urihttp://hdl.handle.net/123456789/95307
dc.languageen
dc.publisherBMC Microbiology
dc.subjectAntibiotic resistance
dc.subjectWater quality
dc.titleComparison of antibiotic resistant Escherichia coli obtained from drinking water sources in northern Tanzania: a cross-sectional study
dc.typeArticle

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