Molecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia

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dc.creatorNyirenda, S. S.
dc.creatorHang’ombe, B. M.
dc.creatorKilonzo, B. S.
dc.creatorKabeta, M. N.
dc.creatorCornellius, M.
dc.creatorSinkala, Y.
dc.date2018-06-20T06:12:32Z
dc.date2018-06-20T06:12:32Z
dc.date2016
dc.date.accessioned2022-10-25T08:51:07Z
dc.date.available2022-10-25T08:51:07Z
dc.descriptionTropical Doctor
dc.descriptionPlague is a re-emerging zoonotic disease caused by the bacterium Yersinia pestis. The disease has caused periodic global devastation since the first outbreak in the 6th century.Twomonths after a suspected plague outbreak inNyimba district, sampleswere collected from94livestock(goats andpigs), 25 rodents, 6 shrews and33 fleas. Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) techniques were used to investigate the presence of Y. pestis, which showed that 16.0% (4/25) of rodents, 16.7% (1/6) of shrews (Crocidura spp) and 6.0% (5/83) of goats were positive for IgG antibodies against Fraction 1 antigen of Y. pestis. Plasminogen activator (Pla) gene (DNA) of Y. pestis was detected in five pools containing 36.4% (12/33) fleas collected from pigs (n¼4), goats (n¼5) and rodents (n¼3). The detection of Pla gene in fleas and IgG antibodies against Fraction1 antigen in rodents, shrews and goats suggest that Y. pestis had been present in the study area in the recent past.
dc.formatapplication/pdf
dc.identifierhttps://www.suaire.sua.ac.tz/handle/123456789/2415
dc.identifier.urihttp://hdl.handle.net/123456789/91048
dc.languageen
dc.publisherSage
dc.subjectDomestic pigs
dc.subjectFleas
dc.subjectGoats
dc.subjectPlague
dc.subjectRodents
dc.subjectZambia
dc.titleMolecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia
dc.typeArticle

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