Production of mouse anti-quail IgY and subsequent labeling with horseradish peroxidase using cyanuric chloride.
| dc.creator | Kassim, Neema | |
| dc.creator | Mtenga, Adelard | |
| dc.creator | Shim, Won-Bo | |
| dc.creator | Chung, Duck-Hwa | |
| dc.date | 2020-05-04T07:35:26Z | |
| dc.date | 2020-05-04T07:35:26Z | |
| dc.date | 2013-04-01 | |
| dc.date.accessioned | 2022-10-25T09:20:30Z | |
| dc.date.available | 2022-10-25T09:20:30Z | |
| dc.description | This research article published by J. Microbiol. Biotechnol, 2013 | |
| dc.description | Polyclonal antibodies labeled with a tracer have been commonly used as secondary antibodies in immunochemical assays to quantify the concentration of antibody-antigen complexes. The majority of these antibodies conjugated with a tracer are commercially available, with the exception of few untouched targets. This study focused on the production and application of mouse anti-quail IgY as an intermediate antibody to link between quail egg yolk IgY and goat anti-mouse IgG-HRP as primary and secondary antibodies, respectively. Subsequently, the produced mouse anti-quail IgY was labeled with horseradish peroxidase (HRP) and its efficiency on enzyme linked immunosorbent assay (ELISA) was compared with that of commercial rabbit anti-chicken IgY-HRP. As an intermediate antibody, mouse anti-quail IgY was successfully produced with good affinity and sensitivity (1:10,000) to the primary and secondary antibodies. Subsequently, mouse anti-quail IgY was effectively conjugated with HRP enzyme, resulting in a secondary antibody with good sensitivity (1:10,000) to quail anti-V. parahaemolyticus and V. vulnificus IgY. The detection limit was 10(5) CFU/ml for both V. parahaemolyticus and V. vulnificus. The efficiency of the produced conjugate to detect quail IgY on ELISA was comparable to that of the commercial rabbit anti-chicken IgY-HRP, and hence the produced and labeled mouse anti-quail IgY-HRP can be used as a secondary antibody to detect any antibody produced in quail. | |
| dc.format | application/pdf | |
| dc.identifier | http://dx.doi.org/10.4014/jmb.1206.06029 | |
| dc.identifier | https://dspace.nm-aist.ac.tz/handle/20.500.12479/741 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/94990 | |
| dc.language | en | |
| dc.publisher | J. Microbiol. Biotechnol | |
| dc.subject | Research Subject Categories::NATURAL SCIENCES | |
| dc.title | Production of mouse anti-quail IgY and subsequent labeling with horseradish peroxidase using cyanuric chloride. | |
| dc.type | Article |