Mycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats

Simple item page
dc.creatorMgode, G. F.
dc.creatorCohen-Bacrie, S.
dc.creatorBedotto, M.
dc.creatorWeetjens, B. J.
dc.creatorCox, C.
dc.creatorJubitana, M.
dc.creatorKuipers, D.
dc.creatorMachang’u, R. S.
dc.creatorKazwala, R.
dc.creatorMfinanga, S.G.
dc.creatorKaufmann, S. H. E.
dc.creatorDrancourt, M.
dc.date2020-06-05T08:41:20Z
dc.date2020-06-05T08:41:20Z
dc.date2015
dc.date.accessioned2022-10-25T08:50:39Z
dc.date.available2022-10-25T08:50:39Z
dc.descriptionArticle of Curr Microbiol (2015) 70:212–218
dc.descriptionTuberculosis (TB) diagnosis in low-income countries is mainly done by microscopy. Hence, little is known about the diversity of Mycobacterium spp. in TB infections. Different genotypes or lineages of Mycobacte- rium tuberculosis vary in virulence and induce different inflammatory and immune responses. Trained Cricetomys rats show a potential for rapid diagnosis of TB. They detect over 28 % of smear-negative, culture-positive TB. How- ever, it is unknown whether these rats can equally detect sputa from patients infected with different genotypes of M. tuberculosis. A 4-month prospective study on diversity of Mycobacterium spp. was conducted in Dar es Salaam, Tanzania. 252 sputa from 161 subjects were cultured on Lowenstein-Jensen medium and thereafter tested by rats. Mycobacterial isolates were subjected to molecular iden- tification and multispacer sequence typing (MST) to determine species and genotypes. A total of 34 Mycobac- terium spp. isolates consisting of 32 M. tuberculosis, 1 M. avium subsp. hominissuis and 1 M. intracellulare were obtained. MST analyses of 26 M. tuberculosis isolates yielded 10 distinct MST genotypes, including 3 new genotypes with two clusters of related patterns not grouped by geographic areas. Genotype MST-67, shared by one- third of M. tuberculosis isolates, was associated with the Mwananyamala clinic. This study shows that diverse M. tuberculosis genotypes (n = 10) occur in Dar es Salaam and trained rats detect 80 % of the genotypes. Sputa with two M. tuberculosis genotypes (20 %), M. avium homin- issuis and M. intracellulare were not detected. Therefore, rats detect sputa with different M. tuberculosis genotypes and can be used to detect TB in resource-poor countries.
dc.formatapplication/pdf
dc.identifierhttps://www.suaire.sua.ac.tz/handle/123456789/3077
dc.identifier.urihttp://hdl.handle.net/123456789/90474
dc.languageen
dc.publisherSpringer Science+Business
dc.subjectMycobacterium genotypes
dc.subjectPulmonary tuberculosis infections
dc.subjectTrained African Giant
dc.subjectPouched Rats
dc.titleMycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats
dc.typeArticle

Files

Collections

Sokoine University