LC-MS analysis with thiol derivatization to differentiate [Dhb7]- from [Mdha7]‑Microcystins: Analysis of cyanobacterial blooms, planktothrix cultures and European crayfish from lake Steinsfjorden, Norway

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dc.creatorMiles, C. O.
dc.creatorSandvik, M.
dc.creatorHaande, S.
dc.creatorNonga, H. E.
dc.creatorBallot, A.
dc.date2017-06-14T13:03:21Z
dc.date2017-06-14T13:03:21Z
dc.date2013-03-26
dc.date.accessioned2022-10-25T08:50:34Z
dc.date.available2022-10-25T08:50:34Z
dc.descriptionEnvironmental Science and Technology 2013, Vol 47: 4080−4087
dc.descriptionKinetic studies showed that [Asp3, Dhb7]MC-RR reacted with mercaptoethanol hundreds of times more slowly than MC-RR and a range of other [Mdha7]-containing microcystin congeners. The difference in reaction rate was sufficiently large that derivatization of microcystin-containing samples with mercaptoethanol, followed by LC-MS analysis, clearly discriminated between microcystins containing the isobaric [Dhb7]- and [Mdha7]-groups. Application of this approach, using LC-MS with both-ion trap and triplequadrupole mass spectrometers, to water samples and Planktothrix cultures from Lake Steinsfjorden, Norway, demonstrated the presence of [Asp3, Dhb7]MC-RR (5), [Asp3]MC-RY (14), and [Asp3]MC-LY (16), as well as analogues tentatively identified as [Asp3]MC-RR (4), [Asp3, DMAdda5, Dhb7]MC-LR (6), [Asp3, Dhb7]MC-HtyR (8), [Asp3]MC-HtyR (9), [Asp3, Dhb7]MC-LR (10), [Asp3]MC-LR (11), [Asp3, Dhb7]MC-RY (15), and [Asp3, Dhb7]MC-LY (17), together with low levels of several other analogues. This is the first use of this thiol-based LC-MS approach to identify Dhb-containing microcystins, and allowed identification of LC-MS peaks in a mixture of [Mdha7]- and [Dhb7]-congeners of [Asp3]MC-RR (4, 5), -RY (14, 15), and -LY (16, 17) in the samples from L. Steinsfjorden. This is also the first report of MC-RY-congeners outside of Africa, or in Planktothrix spp. Analysis of European crayfish (Astacus astacus) taken from L. Steinsfjorden revealed the presence of only trace levels of microcystins in the edible parts.
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dc.identifierhttps://www.suaire.sua.ac.tz/handle/123456789/1591
dc.identifier.urihttp://hdl.handle.net/123456789/90369
dc.languageen
dc.publisherAmerican Chemical Society
dc.subjectThiol Derivatization
dc.subjectCyanobacterial Blooms
dc.subjectPlanktothrix Cultures
dc.subjectEuropean Crayfish
dc.subjectLake Steinsfjorden
dc.titleLC-MS analysis with thiol derivatization to differentiate [Dhb7]- from [Mdha7]‑Microcystins: Analysis of cyanobacterial blooms, planktothrix cultures and European crayfish from lake Steinsfjorden, Norway
dc.typeArticle

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