Development of optimal Culture Method of Sparassis crispa mycelia and new extraction method of Antineoplastic Constituent.

Simple item page
dc.creatorKurosumi, Akihiro
dc.creatorKobayasi, Fumihisa
dc.creatorMtui, Godliving Y. S.
dc.creatorNakamura, Yoshitoshi
dc.date2016-09-21T14:21:45Z
dc.date2016-09-21T14:21:45Z
dc.date2006-05
dc.date.accessioned2018-03-27T09:00:43Z
dc.date.available2018-03-27T09:00:43Z
dc.descriptionThe optimal culture condition of basidiomycete Sparassis crispa mycelia was determined for producing a large amount of antineoplastic constituent, i.e. β-1,3-glucan. Furthermore, a new extraction method with steam explosion as a pretreatment was attempted to increase the amount of antineoplastic constituent extracted from S. crispa mycelia. The shake-flask liquid culture of fungal mycelia could shorten a culture time up to about 1/3 compared with the conventional stationary liquid culture. The productivity of S. crispa mycelia reached its maximum at an initial glucose concentration of 30 g/L, a culture temperature of 25–30 °C, and pH 5. Steam explosion at a steam temperature of 225 °C for 5 min not only enhanced the amount of antineoplastic constituent extracted from the mycelia but also shortened the extraction time significantly.
dc.identifierKurosumi, A., Kobayasi, F., Mtui, G. and Nakamura, Y., 2006. Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent. Biochemical engineering journal, 30(1), pp.109-113.
dc.identifierhttp://hdl.handle.net/20.500.11810/4030
dc.identifier.urihttp://hdl.handle.net/20.500.11810/4030
dc.languageen
dc.subjectBioconversion
dc.subjectFilamentous fungi
dc.subjectβ-1,3-Glucan
dc.subjectMicrobial growth
dc.subjectPlant cell culture
dc.subjectSparassis crispa
dc.titleDevelopment of optimal Culture Method of Sparassis crispa mycelia and new extraction method of Antineoplastic Constituent.
dc.typeJournal Article, Peer Reviewed

Files

Collections

Department of Molecular Biology and Biotechnology