Mycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats

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dc.creatorMgode, G. F.
dc.creatorCohen-Bacrie, S.
dc.creatorBedotto, M.
dc.creatorWeetjens, B. J.
dc.creatorCox, C.
dc.creatorJubitana, M.
dc.creatorKuipers, D.
dc.creatorMachang’u, R. S.
dc.creatorKazwala, R.
dc.creatorMfinanga, S.G.
dc.creatorKaufmann, S. H. E.
dc.creatorDrancourt, M.
dc.date.accessioned2020-06-05T08:41:20Z
dc.date.accessioned2025-08-05T07:19:50Z
dc.date.available2020-06-05T08:41:20Z
dc.date.created2020-06-05T08:41:20Z
dc.date.issued2015
dc.description.abstractTuberculosis (TB) diagnosis in low-income countries is mainly done by microscopy. Hence, little is known about the diversity of Mycobacterium spp. in TB infections. Different genotypes or lineages of Mycobacte- rium tuberculosis vary in virulence and induce different inflammatory and immune responses. Trained Cricetomys rats show a potential for rapid diagnosis of TB. They detect over 28 % of smear-negative, culture-positive TB. How- ever, it is unknown whether these rats can equally detect sputa from patients infected with different genotypes of M. tuberculosis. A 4-month prospective study on diversity of Mycobacterium spp. was conducted in Dar es Salaam, Tanzania. 252 sputa from 161 subjects were cultured on Lowenstein-Jensen medium and thereafter tested by rats. Mycobacterial isolates were subjected to molecular iden- tification and multispacer sequence typing (MST) to determine species and genotypes. A total of 34 Mycobac- terium spp. isolates consisting of 32 M. tuberculosis, 1 M. avium subsp. hominissuis and 1 M. intracellulare were obtained. MST analyses of 26 M. tuberculosis isolates yielded 10 distinct MST genotypes, including 3 new genotypes with two clusters of related patterns not grouped by geographic areas. Genotype MST-67, shared by one- third of M. tuberculosis isolates, was associated with the Mwananyamala clinic. This study shows that diverse M. tuberculosis genotypes (n = 10) occur in Dar es Salaam and trained rats detect 80 % of the genotypes. Sputa with two M. tuberculosis genotypes (20 %), M. avium homin- issuis and M. intracellulare were not detected. Therefore, rats detect sputa with different M. tuberculosis genotypes and can be used to detect TB in resource-poor countries.
dc.identifierhttps://www.suaire.sua.ac.tz/handle/123456789/3077
dc.identifier.urihttp://repository.costech.or.tz/handle/20.500.14732/96980
dc.languageen
dc.publisherSpringer Science+Business
dc.subjectMycobacterium genotypes
dc.subjectPulmonary tuberculosis infections
dc.subjectTrained African Giant
dc.subjectPouched Rats
dc.titleMycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats
dc.typeArticle

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